Department of Chemical and Biomolecular Engineering, Sogang University, Seoul 121-742, Korea.
Nanotechnology. 2013 Sep 13;24(36):365301. doi: 10.1088/0957-4484/24/36/365301. Epub 2013 Aug 13.
We fabricate a nanoscale biomemory device composed of recombinant azurin on nanogap electrodes. For this, size-controllable nanogap electrodes are fabricated by photolithography, electron beam lithography, and surface catalyzed chemical deposition. Moreover, we investigate the effect of gap distance to optimize the size of electrodes for a biomemory device and explore the mechanism of electron transfer from immobilized protein to a nanogap counter-electrode. As the distance of the nanogap electrode is decreased in the nanoscale, the absolute current intensity decreases according to the distance decrement between the electrodes due to direct electron transfer, in contrast with the diffusion phenomenon of a micro-electrode. The biomemory function is achieved on the optimized nanogap electrode. These results demonstrate that the fabricated nanodevice composed of a nanogap electrode and biomaterials provides various advantages such as quantitative control of signals and exclusion of environmental effects such as noise. The proposed bioelectronics device, which could be mass-produced easily, could be applied to construct a nanoscale bioelectronics system composed of a single biomolecule.
我们制作了一种由重组天青蛋白在纳米间隙电极上组成的纳米级生物记忆器件。为此，通过光刻、电子束光刻和表面催化化学沉积制造了尺寸可控的纳米间隙电极。此外，我们研究了间隙距离对优化生物记忆器件电极尺寸的影响，并探讨了固定化蛋白质向纳米间隙对电极的电子转移机制。随着纳米间隙电极距离的减小，由于直接电子转移，根据电极之间的距离减小，绝对电流强度减小，与微电极的扩散现象相反。在优化的纳米间隙电极上实现了生物记忆功能。这些结果表明，由纳米间隙电极和生物材料组成的纳米器件提供了各种优势，例如信号的定量控制以及排除噪声等环境影响。所提出的生物电子器件易于批量生产，可以应用于构建由单个生物分子组成的纳米级生物电子系统。
