Kolber M A, Broschat K O, Landa-Gonzalez B
Department of Medicine, University of Miami School of Medicine, Florida 33101.
FASEB J. 1990 Sep;4(12):3021-7. doi: 10.1096/fasebj.4.12.2394319.
Cellular DNA fragmentation can be induced in many biological instances without plasma membrane damage. The fungal metabolite, cytochalasin B, is capable of modifying numerous cellular functions related to DNA synthesis. In this work it is demonstrated that cytochalasin B is capable of inducing DNA fragmentation in a number of cells lines. This DNA fragmentation occurs before plasma membrane lysis and over a period of hours. Cytochalasin E and villin, agents that act on the microfilaments, also induce DNA fragmentation. Phorbol dibutyrate, a diacylglyceral analog, is able to inhibit cytochalasin B-induced DNA fragmentation in a dose-dependent fashion. These findings support the interpretation that cytochalasin B is inducing DNA fragmentation via its effect on the actin filaments.
在许多生物学情况下，细胞DNA片段化可在没有质膜损伤的情况下被诱导。真菌代谢产物细胞松弛素B能够改变许多与DNA合成相关的细胞功能。在这项研究中，证明了细胞松弛素B能够在多种细胞系中诱导DNA片段化。这种DNA片段化发生在质膜裂解之前，且持续数小时。作用于微丝的药物细胞松弛素E和绒毛蛋白也能诱导DNA片段化。佛波酯二丁酸酯，一种二酰甘油类似物，能够以剂量依赖的方式抑制细胞松弛素B诱导的DNA片段化。这些发现支持了细胞松弛素B通过其对肌动蛋白丝的作用诱导DNA片段化的解释。
