Shabanpoor Fazel, Hossain Mohammed Akhter, Lin Feng, Wade John D
Florey Neuroscience Institutes, School of Chemistry, The University of Melbourne, Parkville, VIC, Australia.
Methods Mol Biol. 2013;1047:81-7. doi: 10.1007/978-1-62703-544-6_5.
Numerous methods have been developed for the formation of disulfide bonds in recombinant DNA-derived and chemically synthesized peptides and proteins, but only a few have found widespread acceptance. The choice of method(s) for formation of disulfide in synthetic peptides and proteins needs to be tailored for each individual polypeptide in such a way so that the reaction conditions are selective, efficient, and safe and give the maximum yield. Here we describe the sequential formation of three disulfide bonds regioselectively which has been optimized for the synthesis of two-chained, heterodimeric polypeptide members of the insulin-relaxin superfamily.
已经开发出许多方法用于在重组DNA衍生的和化学合成的肽及蛋白质中形成二硫键，但只有少数方法得到广泛认可。在合成肽和蛋白质中形成二硫键的方法选择需要针对每个多肽进行定制，以使反应条件具有选择性、高效性和安全性，并获得最大产率。在此，我们描述了区域选择性地依次形成三个二硫键，该方法已针对胰岛素-松弛素超家族的双链异源二聚体多肽成员的合成进行了优化。
