Annis I, Hargittai B, Barany G
Department of Chemistry, University of Minnesota, Minneapolis 55455, USA.
Methods Enzymol. 1997;289:198-221. doi: 10.1016/s0076-6879(97)89049-0.
The goal of this review has been to present different chemical approaches for the formation of disulfide bonds in synthetic peptides and small proteins. Three general types of approaches have been described: (1) oxidation starting from the unprotected thiols; (2) oxidation starting from protected thiols; and (3) directed methods for formation of unsymmetrical disulfides. Individual or sequential disulfide-forming reactions can be carried out in solution or on a polymeric support. Overall yields and purities of products depends on protecting group combinations chosen, precise reaction conditions, and the targeted structure. Although no procedure can be guaranteed to give outstanding results for all cases, there are sufficient options available to support an optimistic view that one or more approaches can be optimized.
本综述的目的是介绍在合成肽和小蛋白质中形成二硫键的不同化学方法。已描述了三种一般类型的方法：(1) 从未保护的硫醇开始氧化；(2) 从保护的硫醇开始氧化；以及(3) 形成不对称二硫键的定向方法。单个或连续的二硫键形成反应可以在溶液中或在聚合物载体上进行。产物的总产率和纯度取决于所选择的保护基团组合、精确的反应条件和目标结构。尽管不能保证任何程序在所有情况下都能给出出色的结果，但有足够的选择来支持一种乐观的观点，即可以优化一种或多种方法。
