Nair P P, Shami S, Sainz E, Menon M, Jerabek L B, Jones D Y, Judd J T, Campbell W S, Schiffman M H, Taylor P R
Lipid Nutrition Laboratory, Beltsville Human Nutrition Research Center, ARS, USDA, MD 20705.
Int J Cancer. 1990 Sep 15;46(3):374-7. doi: 10.1002/ijc.2910460306.
A dietary intervention study was conducted on 31 premenopausal women (age: 20-40 years) to investigate the relationship between dietary fat and fecal mutagenicity. After a free-living period (baseline) of one menstrual cycle, the subjects were placed on a high-fat diet (40% calories from fat) for 4 menstrual cycles, followed by a low-fat diet (20% calories from fat) for 4 menstrual cycles. One-half of the subjects were randomly assigned throughout the study to a diet with a P:S ratio of 1.0 while the other half was assigned to one with a P:S ratio of 0.3; body weight by group remained constant. Three-day stool samples were collected at the mid-follicular period during the free-living phase and during the 4th menstrual cycle of each of the 2 controlled diet periods. Mutagenicity was assayed by the SOS chromotest. Reduction of dietary fat was accompanied by a significant decrease in fecal mutagenicity in both P:S groups. Combined values, i.e., both P:S groups, were 20.3 units for high-fat diets vs. 8.78 for low-fat diets.
对31名绝经前女性（年龄：20 - 40岁）进行了一项饮食干预研究，以调查膳食脂肪与粪便致突变性之间的关系。在经历一个月经周期的自由生活期（基线期）后，受试者接受了4个月经周期的高脂肪饮食（脂肪提供40%的热量），随后是4个月经周期的低脂肪饮食（脂肪提供20%的热量）。在整个研究过程中，一半受试者被随机分配到P:S比值为1.0的饮食组，另一半被分配到P:S比值为0.3的饮食组；各小组的体重保持恒定。在自由生活期的卵泡中期以及两个控制饮食期的每个阶段的第4个月经周期收集三天的粪便样本。通过SOS显色试验测定致突变性。在两个P:S组中，膳食脂肪的减少均伴随着粪便致突变性的显著降低。合并值，即两个P:S组，高脂肪饮食为20.3单位，低脂肪饮食为8.78单位。
