Pfeil R M, Venkat J A, Plimmer J R, Sham S, Davis K, Nair P P
Environmental Chemistry Laboratory, U.S. Department of Agriculture, Beltsville, Maryland 20705.
Arch Environ Contam Toxicol. 1994 Feb;26(2):201-7. doi: 10.1007/BF00224805.
The genotoxicity of groundwater was evaluated, using a novel application of the SOS microplate assay (SOSMA). Organic residues were extracted from groundwater samples from Maryland, Pennsylvania, and Delaware by using C-18 bonded silica solid phase extraction tubes. Total organic carbon content (TOC) of water samples was also determined. The genotoxicity of the extracts was determined by the SOSMA. Relative activity (RA) as determined by the SOSMA is a quantitative measure of genotoxicity based on a comparison to the activity of the mutagen, 4-nitroquinoline oxide. Low levels of RA (about 2x background) were detected in waters from sites within these states. There was considerable temporal and spatial variation in the observed RA, but no definite patterns were observed in the variation. Between sampling sites there was a positive correlation between RA and TOC; however, this relationship appeared to be reversed occasionally within a sampling site. The extraction and bioassay methods provide an easy and relatively inexpensive means of determining water quality.
采用SOS微孔板分析法（SOSMA）的新应用对地下水的遗传毒性进行了评估。使用C-18键合硅胶固相萃取管从马里兰州、宾夕法尼亚州和特拉华州的地下水样品中提取有机残留物。还测定了水样的总有机碳含量（TOC）。提取物的遗传毒性通过SOSMA测定。SOSMA测定的相对活性（RA）是基于与诱变剂4-硝基喹啉氧化物活性的比较对遗传毒性进行的定量测量。在这些州内各地点的水体中检测到低水平的RA（约为背景值的2倍）。观察到的RA存在相当大的时间和空间变化，但未观察到变化的明确模式。在采样点之间，RA与TOC呈正相关；然而，在一个采样点内这种关系偶尔会出现逆转。提取和生物测定方法提供了一种简单且相对廉价的水质测定方法。
