依地酸处理并洗涤过的大鼠血小板的光散射变化：二磷酸腺苷和钙离子在冷藏影响方面的作用。
Light scattering changes of edetic acid-treated, washed rat platelets: effect of adenosine diphosphate and calcium ion in relation to chilling.
作者信息
Shibata S, Kobayashi B
出版信息
Thromb Diath Haemorrh. 1975 Jun 30;33(3):508-18.
The effe-ts of adenosine diphosphate (ADP) and Ca2+ on platelet morphology were studied in edetic acid (EDTA)-treated, washed platelets of rat by means of light scattering measurement at right angles. ADP caused a transient change of light scattering, which was independent of concentration above 5 muM and of pH between 4 and 10. When the platelets were pretreated with ADP or chilled persistently, Ca2+ also caused another transient change of light scattering. The effect of Ca2+ was abolsihed at pH below 6.5. The velocity of both ADP- and Ca2+-induced response was dependnet on temperature, and the magnitude was reduced by metabolic inhibitors and SH-blocking agents. Adenosine triphosphate (ATP) competitively inhibited the ADP-induced response, but not the Ca2+-induced one. The response to Ca2+ of the control and chilled platelets was compared by scanning electron microgram. Apparent similarity of the effect of chilling on membrane structure to that of ADP was suggested.
通过直角光散射测量法，研究了二磷酸腺苷（ADP）和Ca2+对乙二胺四乙酸（EDTA）处理的大鼠洗涤血小板形态的影响。ADP引起光散射的瞬态变化，该变化在浓度高于5μM以及pH值在4至10之间时与浓度无关。当血小板用ADP预处理或持续冷藏时，Ca2+也会引起光散射的另一种瞬态变化。Ca2+的作用在pH值低于6.5时消失。ADP和Ca2+诱导反应的速度均取决于温度，且其幅度会被代谢抑制剂和SH阻断剂降低。三磷酸腺苷（ATP）竞争性抑制ADP诱导的反应，但不抑制Ca2+诱导的反应。通过扫描电子显微镜比较了对照血小板和冷藏血小板对Ca2+的反应。结果表明，冷藏对膜结构的影响与ADP的影响明显相似。
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