Ts'ao C
Am J Pathol. 1976 Dec;85(3):581-94.
This paper describes the aggregation of rat platelets by adenosine triphosphate (ATP). The aggregometry of ATP-induced aggregation and the ultrastructure of ATP-aggregated platelets were compared and contrasted with those of adenosine diphosphate (ADP)-treated and collagen-treated samples. Human platelets were also studied alongside with rat specimens. Several lines of evidence indicate that the ATP-induced aggregation of rat platelet-rich plasma (PRP) is not a result of contaminating ADP in the ATP preparation. ATP did not cause aggregation of human platelets; it inhibited ADP- and collagen-induced human platelet aggregation. ATP pretreated with a creatine phosphate/creatine phosphokinase system caused similar rat platelet aggregation as did ATP not treated with this system. The aggregometry of ATP-induced aggregation of rat PRP was similar to that of collagen-induced aggregation but markedly different from that of ADP-induced aggregation. However, the nature of ATP-induced aggregation was similar to that induced by ADP. Both ATP- and ADP-induced rat platelet aggregations were not affected by adenosine, adenosine monophosphate, or acetylsalicylic acid. The ultrastructure of ATP-aggregated platelets was similar to that of ADP-aggregated ones. It appears that either platelets of rats possess specific ATP receptors or the rat plasma contains a material, lacking or insufficiently present in human plasma, that converts ATP to ADP in a fashion similar to the release of ADP from platelet storage granules.
本文描述了三磷酸腺苷（ATP）诱导大鼠血小板聚集的情况。将ATP诱导聚集的血小板聚集测定法以及ATP聚集血小板的超微结构与二磷酸腺苷（ADP）处理和胶原处理样本的情况进行了比较和对比。同时还对人类血小板与大鼠样本进行了研究。多条证据表明，ATP诱导富含大鼠血小板血浆（PRP）的聚集并非ATP制剂中污染的ADP所致。ATP不会引起人类血小板聚集；它会抑制ADP和胶原诱导的人类血小板聚集。用磷酸肌酸/磷酸肌酸激酶系统预处理的ATP与未用该系统处理的ATP引起的大鼠血小板聚集相似。ATP诱导大鼠PRP聚集的血小板聚集测定法与胶原诱导聚集的情况相似，但与ADP诱导聚集的情况明显不同。然而，ATP诱导聚集的性质与ADP诱导的相似。ATP和ADP诱导的大鼠血小板聚集均不受腺苷、一磷酸腺苷或乙酰水杨酸的影响。ATP聚集血小板的超微结构与ADP聚集血小板的超微结构相似。似乎大鼠血小板要么具有特定的ATP受体，要么大鼠血浆中含有一种在人类血浆中缺乏或含量不足的物质，该物质以类似于从血小板储存颗粒中释放ADP的方式将ATP转化为ADP。
