Kohda Tetsuya, Sakuma Satoru, Abe Muneyuki, Fujimoto Yohko
Laboratory of Physiological Chemistry, Osaka University of Pharmaceutical Sciences, Takatsuki, Osaka, Japan.
Cell Biochem Funct. 2014 Mar;32(2):188-93. doi: 10.1002/cbf.2992. Epub 2013 Aug 15.
The aim of this study was to assess a possible role of monochloramine (NH2 Cl), one of the reactive chlorine species, which induce oxidative stress, on the proliferation of colorectal cancer cell line Caco-2. At concentrations ranging from 10 to 200 μM, NH2 Cl (14-61% inhibition), but not hypochlorous acid, dose-dependently inhibited the cell viability of Caco-2 cells. Experiments utilizing methionine (a scavenger of NH2 Cl), taurine-chloramine and glutamine-chloramine revealed that only NH2 Cl affects the cancer cell proliferation among reactive chlorine species, with a relative specificity. Furthermore, flow-cytometry experiments showed that the anti-proliferative effect of NH2 Cl is partially attributable to both apoptosis and G2/M cell cycle arrest. These results suggest that NH2 Cl has the potential to suppress colorectal cancer cell proliferation.
本研究的目的是评估活性氯物种之一的一氯胺（NH2Cl）对结肠癌细胞系Caco-2增殖的可能作用，活性氯物种会诱导氧化应激。在10至200μM的浓度范围内，NH2Cl（抑制率为14 - 61%）而非次氯酸，呈剂量依赖性地抑制Caco-2细胞的活力。利用蛋氨酸（NH2Cl的清除剂）、牛磺酸氯胺和谷氨酰胺氯胺进行的实验表明，在活性氯物种中，只有NH2Cl以相对特异性的方式影响癌细胞增殖。此外，流式细胞术实验表明，NH2Cl的抗增殖作用部分归因于细胞凋亡和G2/M期细胞周期阻滞。这些结果表明，NH2Cl具有抑制结肠癌细胞增殖的潜力。
