Inaba Tohru, Yuasa Soichi, Nakanishi Masaki, Takahashi Mayako, Taniguchi Hiroshi, Saitoh Kensuke, Oku Narihiro, Fujita Naohisa
Department of Infection Control and Laboratory Medicine, Kyoto Prefectural University of Medicine, Kyoto 602 8566, Japan.
Rinsho Byori. 2013 Jun;61(6):482-7.
We collected the different volumes of venous blood(60 microL, 125 microL, 250 microL and 500 microL) into micro sample cups from respective volunteer to compare complete blood count (CBC) among 4 sample volumes, and found that 60 microL of sample volume seemed enough for CBC measurement using Microsemi LC-667 CRP (Horiba Co.). Subsequently, we measured CBC using 60 microL of peripheral blood after combining one of the 3 capillary tubes (heparin coated, EDTA coated and plain) with either EDTA coated or plain micro sample cups to examine the effect of anticoagulants contained into these commercially available maneuvers for capillary blood sampling. When we used the plain micro sample cup, platelet aggregation and false increase of white blood cell(WBC) count were observed irrespective to the combination of capillary tubes. We also tried whether commercially recommended volume (250 microL) of sample could be obtained by either fingertip or earlobe puncture from volunteers, and found that 7 of 16 fingertip and only 1 earlobe punctures could achieve sufficient volume. Whereas, at least 60 microL of sample were available more than 80% of volunteers by both methods, and CBC data obtained from these lesser samples obtained by fingertip puncture showed no statistically significant differences when compared with those of conventional venous samples (2 mL). From these findings, we concluded that at least 60 microL of capillary blood obtained from fingertip then collected into EDTA coated micro sample cup was enough to measure CBC using Microsemi LC-667 CRP.
我们从各个志愿者身上采集不同体积的静脉血（60微升、125微升、250微升和500微升）到微量样本杯中，以比较4种样本体积下的全血细胞计数（CBC），发现60微升的样本体积似乎足以使用Microsemi LC - 667 CRP（堀场公司）进行CBC测量。随后，我们将3种毛细管（肝素涂层、乙二胺四乙酸（EDTA）涂层和普通毛细管）之一与EDTA涂层或普通微量样本杯组合后，使用60微升外周血测量CBC，以检查这些市售毛细管采血操作中所含抗凝剂的效果。当我们使用普通微量样本杯时，无论毛细管的组合如何，均观察到血小板聚集和白细胞（WBC）计数的假性增加。我们还尝试能否通过对志愿者进行指尖或耳垂穿刺获得市售推荐体积（250微升）的样本，发现16次指尖穿刺中有7次、耳垂穿刺仅有1次能够获得足够的体积。然而，两种方法对超过80%的志愿者均可获得至少60微升的样本，并且与传统静脉样本（2毫升）相比，通过指尖穿刺获得的这些较少样本所得到的CBC数据无统计学显著差异。基于这些发现，我们得出结论，从指尖采集至少60微升毛细血管血，然后收集到EDTA涂层微量样本杯中，足以使用Microsemi LC - 6,67 CRP测量CBC。
