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Wnt5a 表达与膀胱尿路上皮癌的组织病理学分级/分期的相关性。

Correlation of Wnt5a expression with histopathological grade/stage in urothelial carcinoma of the bladder.

机构信息

Department of Biomedical Sciences, Heritage College of Osteopathic Medicine, Ohio University, Athens, OH 45701-2979, USA.

出版信息

Diagn Pathol. 2013 Aug 15;8:139. doi: 10.1186/1746-1596-8-139.

DOI:10.1186/1746-1596-8-139
PMID:23947922
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3846281/
Abstract

BACKGROUND

Bladder cancer, including urothelial carcinoma (UC), is the most common malignancy of the urinary tract and the fourth most frequent cancer overall in men. Wnt5a, a member of the Wnt family of proteins, has been shown to have contradictory roles in the pathogenesis of many cancers, acting either as tumor suppressor or tumor promoter. The objective of this study was to investigate the expression and role of Wnt5a in the pathogenesis of UC and suggest possible clinical applications for diagnosis, prognosis and treatment.

METHODS

We characterized the expression of Wnt5a in 33 human UC samples using immunohistochemistry. The samples were obtained via transurethral resection, immediately fixed in formalin and then embedded in paraffin. The correlation between Wnt5a immunoreactivity, histological grade, and pathological stage of the tumor was analyzed. The expression of Wnt5a mRNA as well as the effect of Wnt5a on cell migration was also evaluated in two UC cell lines, T24 and J82, and a normal urothelial cell line.

RESULTS

Our immunohistochemical results revealed that Wnt5a staining intensity correlated positively with the histological grade and pathological stage of the UC. Wnt5a mRNA expression differed widely in the three urothelial cell lines, with high levels in one carcinoma cell line and low levels in the other cell line in comparison to the normal urothelial cell line. Migration increased in both UC cell lines in response to Wnt5a treatment.

CONCLUSIONS

Our results show that the Wnt5a pathway may play a role in the pathogenesis of UC and suggest that Wnt5a may serve as an additional, complementary diagnostic/prognostic marker for UC.

VIRTUAL SLIDE

http://www.diagnosticpathology.diagnomx.eu/vs/1952312091979566.

摘要

背景

膀胱癌，包括尿路上皮癌（UC），是最常见的泌尿道恶性肿瘤，也是男性中第四最常见的癌症。Wnt5a 是 Wnt 蛋白家族的成员，其在许多癌症的发病机制中具有矛盾的作用，既可以作为肿瘤抑制因子，也可以作为肿瘤促进因子。本研究旨在探讨 Wnt5a 在 UC 发病机制中的表达和作用，并为诊断、预后和治疗提供可能的临床应用。

方法

我们使用免疫组织化学方法对 33 例人 UC 样本中的 Wnt5a 表达进行了特征描述。这些样本通过经尿道切除术获得，立即固定在福尔马林中，然后嵌入石蜡中。分析了 Wnt5a 免疫反应性与肿瘤的组织学分级和病理分期之间的相关性。还评估了 Wnt5a 在两种 UC 细胞系 T24 和 J82 以及正常尿路上皮细胞系中的 mRNA 表达以及 Wnt5a 对细胞迁移的影响。

结果

我们的免疫组织化学结果显示，Wnt5a 染色强度与 UC 的组织学分级和病理分期呈正相关。三种尿路上皮细胞系中的 Wnt5a mRNA 表达差异很大，与正常尿路上皮细胞系相比，一种癌细胞系中的表达水平较高，而另一种细胞系中的表达水平较低。Wnt5a 处理后，两种 UC 细胞系的迁移均增加。

结论

我们的研究结果表明，Wnt5a 通路可能在 UC 的发病机制中发挥作用，并表明 Wnt5a 可能作为 UC 的另一个补充诊断/预后标志物。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6f98/3846281/29dce8e6f640/1746-1596-8-139-6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6f98/3846281/521be4314c2d/1746-1596-8-139-1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6f98/3846281/a324dab2d6bd/1746-1596-8-139-2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6f98/3846281/97f34330a004/1746-1596-8-139-3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6f98/3846281/4b135fd41384/1746-1596-8-139-4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6f98/3846281/80aa835a68ec/1746-1596-8-139-5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6f98/3846281/29dce8e6f640/1746-1596-8-139-6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6f98/3846281/521be4314c2d/1746-1596-8-139-1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6f98/3846281/a324dab2d6bd/1746-1596-8-139-2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6f98/3846281/97f34330a004/1746-1596-8-139-3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6f98/3846281/4b135fd41384/1746-1596-8-139-4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6f98/3846281/80aa835a68ec/1746-1596-8-139-5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6f98/3846281/29dce8e6f640/1746-1596-8-139-6.jpg

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