TU Dresden, International Institute Zittau, Department of Bio- and Environmental Sciences, Markt 23, 02763 Zittau, Germany; University of Buenos Aires, Faculty of Exact and Natural Sciences, Department of Biodiversity and Experimental Biology, Intendente Güiraldes 2160, Ciudad Universitaria, C1428EGA, Argentina.
J Biotechnol. 2013 Oct 10;168(1):15-23. doi: 10.1016/j.jbiotec.2013.08.007. Epub 2013 Aug 12.
The present work was carried out with the aim to analyze the secretome of Trametes versicolor BAFC 2234 grown on tomato juice medium supplemented with copper and manganese. T. versicolor BAFC 2234 was selected among diverse wood dwelling agaricomycetes from Argentina by its ability to cause a strong white rot on hardwood and in addition to show high tolerance toward phenolic compounds. A considerable number of the identified proteins were related to the degradation/modification of lignocelluloses. Hydrolases, peroxidases and phenoloxidases were the most abundant enzymes produced under the above-mentioned culture conditions. The lignin-modifying oxidoreductases laccase, manganese peroxidase (MnP) and versatile peroxidase (VP) were successfully purified - the latter for the first time from T. versicolor. The native VP protein has a molecular mass of 45kDa and an isoelectric point of pH 3.7. The study clearly shows that complex plant-based media being rich in phenolics, such as tomato juice, can stimulate the secretion of a broad set of extracellular lignocellulolytic enzymes. Using such natural products as fungal culture media may give the opportunity to investigate plant biomass decomposition as well as the biodegradation of organic pollutants in an environment close to nature.
本研究旨在分析在添加铜和锰的番茄汁培养基上生长的彩绒革盖菌（Trametes versicolor）BAFC 2234 的胞外分泌物。彩绒革盖菌（Trametes versicolor）BAFC 2234 是从阿根廷不同的木栖伞菌中筛选出来的，因其能够在硬木上引起强烈的白色腐烂，并且对酚类化合物表现出很高的耐受性。鉴定出的大量蛋白质与木质纤维素的降解/修饰有关。在上述培养条件下，产酶最多的是水解酶、过氧化物酶和酚氧化酶。木质素修饰氧化还原酶漆酶、锰过氧化物酶（MnP）和多功能过氧化物酶（VP）被成功地纯化，后者是首次从彩绒革盖菌中纯化得到。天然 VP 蛋白的分子量为 45kDa，等电点为 pH3.7。研究清楚地表明，富含酚类物质的复杂植物培养基，如番茄汁，可以刺激一组广泛的木质纤维素降解酶的分泌。使用真菌培养基等天然产物，为研究植物生物质分解以及在更接近自然环境的条件下有机污染物的生物降解提供了机会。
