Department of Environmental Protection, Estación Experimental del Zaidín, Consejo Superior de Investigaciones Cientificas (EEZ-CSIC), C/Profesor Albareda 1, 18008 Granada, Spain.
Department of Environmental Protection, Estación Experimental del Zaidín, Consejo Superior de Investigaciones Cientificas (EEZ-CSIC), C/Profesor Albareda 1, 18008 Granada, Spain.
Bioresour Technol. 2013 Oct;146:345-354. doi: 10.1016/j.biortech.2013.07.093. Epub 2013 Jul 25.
Scarce information is available on the changes in abundance of microbial taxa during vermicomposting. Quantitative PCR and DGGE analysis were used to monitor variations in the microbial structure, relative abundance of four bacterial classes and fungi over the vermicomposting and maturation period of wet olive cake (O) and vine shoots (W). Multivariate correlation analysis between microbial structure and abundance, earthworm biomass and enzyme activities revealed similar and divergent interactions in both processes. Although Eisenia fetida development was different, significant correlations were found with β-glucosidase activity and with bacterial and fungal structure. In the vermicomposting period of O and W, a decline was found in bacteria (94% and 77%), fungi (93% and 94%), and Gammaproteobacteria (56% and 71%) but an increase in Betaproteobacteria and Actinobacteria (62-79%). Alphaproteobacteria increased only in O (26%). Despite the different initial lignocellulose wastes, the mature vermicomposts were similar in microbial and biochemical properties.
有关蚯蚓堆肥过程中微生物类群丰度变化的信息很少。本研究采用定量 PCR 和 DGGE 分析方法，监测了湿橄榄饼（O）和葡萄藤（W）在蚯蚓堆肥和成熟过程中微生物结构、四类细菌和真菌相对丰度的变化。微生物结构和丰度、蚯蚓生物量和酶活性之间的多元相关分析表明，这两个过程存在相似和不同的相互作用。尽管赤子爱胜蚓的生长情况不同，但与β-葡萄糖苷酶活性以及细菌和真菌结构均呈显著相关。在 O 和 W 的蚯蚓堆肥期，细菌（94%和 77%）、真菌（93%和 94%）和γ-变形菌（56%和 71%）数量下降，但β-变形菌和放线菌数量增加（62-79%）。α-变形菌仅在 O 中增加（26%）。尽管初始木质纤维素废物不同，但成熟的蚯蚓堆肥在微生物和生化特性方面相似。
