Zhao Jie, Liu Yali, Jian Qiang, Li Juan, Chi Sumin
Department of Physiology, Fourth Military Medical University, Xi'an, China.
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2013 Aug;29(8):813-7.
To investigate the effects of Rab23 over-expression and Rab23 knock-out on the migration and invasion of breast cancer cell line Bcap-37.
Western blotting was employed to detect the expression of Rab23 in breast cell line HBL-100 and cancer cell lines Bcap-37, MDA-MB-231, and MCF-7. Lentiviral vectors with Rab23 genes and with Rab23 RNAi were respectively transfected into breast cancer cell line Bcap-37, and the cells presenting the stable over-expression of Rab23 and the knock-out of Rab23 were respectively selected. Scratch healing assay and Transwell(R); invasion assay were used to examine the alterations of Bcap-37 cell migration and invasion potentials after Rab23 over-expression and Rab23 knock-out.
Western blotting indicated that Rab23 was expressed in all breast cancer cell lines and normal breast cell line. And Rab23 expression level was the highest in Bcap-37 cells, which was statistically different as compared with HBL-100 cells (P<0.01). Compared with the control group, Bcap-37 cell line with Rab23 over-expression had the dramatically enhanced migration and invasion potentials (P<0.01). On the other hand, Bcap-37 cell line with Rab23 knock-out showed the significantly attenuated migration and invasion potentials (P<0.01).
Rab23 plays a pivotal role in the migration and invasion of Bcap-37 cells.
研究Rab23过表达和敲除对乳腺癌细胞系Bcap-37迁移和侵袭的影响。
采用蛋白质免疫印迹法检测Rab23在乳腺细胞系HBL-100及癌细胞系Bcap-37、MDA-MB-231和MCF-7中的表达。将携带Rab23基因和Rab23 RNA干扰的慢病毒载体分别转染至乳腺癌细胞系Bcap-37，分别筛选出Rab23稳定过表达和敲除的细胞。采用划痕愈合试验和Transwell侵袭试验检测Rab23过表达和敲除后Bcap-37细胞迁移和侵袭能力的变化。
蛋白质免疫印迹法显示，Rab23在所有乳腺癌细胞系和正常乳腺细胞系中均有表达。且Rab23在Bcap-37细胞中的表达水平最高，与HBL-100细胞相比差异有统计学意义（P<0.01）。与对照组相比，Rab23过表达的Bcap-37细胞系迁移和侵袭能力显著增强（P<0.01）。另一方面，Rab23敲除的Bcap-37细胞系迁移和侵袭能力明显减弱（P<0.01）。
Rab23在Bcap-37细胞的迁移和侵袭中起关键作用。
