Wang Zhigao, Halida Yasen, Zhao Fang, Shi Wei, Ma Xiumin, Xu Qi, Guo Xinhong
Department of Hematology, Xinjiang Medical University, Urumqi, China.
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2013 Aug;29(8):850-3.
To investigate CD28 expression on CD4(+);T cells and its relationship with IFN-γ/IL-10 ration in patients with primary immune thrombocytopenia (ITP) before and after treatment.
The expression of CD4(+);CD28(+); was detected by flow cytometry, the levels of IFN-γ and IL-10 were determined by double-antibody sandwich ELISA and platelet count was tested by blood cells automatical counter in peripheral blood of 30 patients with ITP before and after glucocorticoid treatment and 26 cases of normal controls. Then the correlations between the outcomes were analyzed.
The expression of CD4(+);CD28(+); of ITP patients before treatment was higher than that of the normal control group (P<0.05), however, after treatment the expression between the two groups had no statistical difference (P>0.05). Compared with the control group, the ITP patients before treatment showed the significantly higher level of IFN-γ and the significantly decreased level of IL-10, and the ratio of IFN-γ/IL-10 was significantly raised (P<0.01); while ITP patients after treatment had no statistically significant difference in the above indexes from the normal controls (P>0.05). Moreover, CD4(+);CD28(+); was positively correlated with IFN-γ/IL-10 ratio (P<0.05), and was negatively correlated with platelet count in ITP patients before treatment (P<0.05).
Co-stimulatory molecule CD4(+);CD28(+); was closely related with immune disorder of ITP. It maybe played a role in the pathogenesis of ITP through involving Th1 advantage state formation.
探讨原发性免疫性血小板减少症（ITP）患者治疗前后CD4(+)T细胞上CD28的表达及其与IFN-γ/IL-10比值的关系。
采用流式细胞术检测30例ITP患者糖皮质激素治疗前后及26例正常对照者外周血中CD4(+)CD28(+)的表达，双抗体夹心ELISA法测定IFN-γ和IL-10水平，血细胞自动分析仪检测血小板计数。然后分析各结果之间的相关性。
ITP患者治疗前CD4(+)CD28(+)的表达高于正常对照组（P<0.05），但治疗后两组间表达无统计学差异（P>0.05）。与对照组相比，ITP患者治疗前IFN-γ水平显著升高，IL-10水平显著降低，IFN-γ/IL-10比值显著升高（P<0.01）；而ITP患者治疗后上述指标与正常对照组无统计学差异（P>0.05）。此外，ITP患者治疗前CD4(+)CD28(+)与IFN-γ/IL-10比值呈正相关（P<0.05），与血小板计数呈负相关（P<0.05）。
共刺激分子CD4(+)CD28(+)与ITP的免疫紊乱密切相关。它可能通过参与Th1优势状态的形成在ITP的发病机制中起作用。
