Li Ran, Qu Wen, Fu Rong, Wang Guo-jin, Wang Xiao-ming, Wang Hua-quan, Li Li-juan, Liu Hong, Wu Yu-hong, Xing Li-min, Guan Jing, Liu Hui, Shao Zong-hong
Department of Hematology, General Hospital, Tianjin Medical University, Tianjin 300052, China.
Zhonghua Yi Xue Za Zhi. 2013 Jun 11;93(22):1696-9.
To explore the relationship between the dendritic cell (DC) subsets and abnormal expression of transcription factors Gata-3 and T-bet in patients with immune thrombocytopenia (ITP).
The plasmacytoid DC (pDC) and myeloid DC (mDC) of 33 ITP (16 untreated, 17 remitted) patients and 12 healthy controls were analyzed by flow cytometry (FCM) . The expressions of Gata-3 mRNA and T-bet mRNA in peripheral blood mononuclear cell (PBMNC) were detected by reverse transcription-polymerase chain reaction (RT-PCR) .The levels of interleukin-4 (IL-4) and interferon-gamma (IFN-γ) were measured by FCM in 33 ITP patients and 12 healthy controls.
The percentage of pDC in PBMNC was 0.49% ± 0.18% in untreated and it was higher than that in remitted ITP patients (0.27% ± 0.17%) and in controls (0.32% ± 0.13%) (both P < 0.05). The percentage of mDC in PBMNC was 0.23% ± 0.17% in untreated, which was lower than that in remitted ITP patients (0.33% ± 0.18)% and in controls (0.31% ± 0.11%), but no statistic difference in mDC expression existed among 3 groups (P > 0.05). pDC/mDC ratios was (3.15 ± 2.01) in untreated ITP patients and it was higher than that in remitted ITP patients (0.81 ± 0.32) and in controls (1.07 ± 0.44) (both P < 0.05). The relative mRNA expression levels of Gata-3 were 2775 ± 489, 1357 ± 307 and 652 ± 165 respectively. And the expression of Gata-3mRNA in untreated group was higher than that in remission group or healthy controls (both P < 0.05). The relative mRNA expression levels of T-bet were 782 ± 394, 583 ± 176 and 576 ± 120. No statistic difference in T-bet expression existed among 3 groups (P > 0.05). Gata-3mRNA/T-bet mRNA ratio was (4.13 ± 1.69 ) in untreated group and it was higher than that of remission group (2.45 ± 0.69) or controls (1.15 ± 0.27) (both P < 0.05). The level of IL-4 in the untreated group was 9.14% ± 4.34% and it was higher than that of remission group (4.78% ± 1.69%) or controls (4.86% ± 1.41%). The level of IFN-γ in the untreated group was lower than that of controls (P < 0.05). Significant positive correlations existed between Gata-3 and pDC/mDC ratio (r = 0.585, P < 0.01). Significant positive correlations existed between Gata-3 and IL-4 ( r = 0.463, P < 0.05).
The mechanism of ITP may be due to a disorder of DC subsets and a high expression of Gata-3.
探讨免疫性血小板减少症（ITP）患者树突状细胞（DC）亚群与转录因子Gata-3和T-bet异常表达之间的关系。
采用流式细胞术（FCM）分析33例ITP患者（16例未治疗、17例缓解期）及12例健康对照者的浆细胞样DC（pDC）和髓样DC（mDC）。采用逆转录-聚合酶链反应（RT-PCR）检测外周血单个核细胞（PBMNC）中Gata-3 mRNA和T-bet mRNA的表达。采用FCM检测33例ITP患者和12例健康对照者的白细胞介素-4（IL-4）和干扰素-γ（IFN-γ）水平。
未治疗的ITP患者PBMNC中pDC百分比为0.49%±0.18%，高于缓解期ITP患者（0.27%±0.17%）和对照组（0.32%±0.13%）（均P<０.０５）。未治疗的ITP患者PBMNC中mDC百分比为0.23%±0.17%，低于缓解期ITP患者（0.33%±0.18%）和对照组（0.31%±0.11%），但3组间mDC表达差异无统计学意义（P>0.05）。未治疗的ITP患者pDC/mDC比值为（3.15±2.01），高于缓解期ITP患者（0.81±0.32）和对照组（1.07±0.44）（均P<０.０５）。Gata-3的相对mRNA表达水平分别为2775±489、1357±307和652±165。未治疗组Gata-3mRNA表达高于缓解组和健康对照组（均P<０.０５）。T-bet的相对mRNA表达水平分别为782±394、583±176和576±120。3组间T-bet表达差异无统计学意义（P>0.05）。未治疗组Gata-3mRNA/T-bet mRNA比值为（4.13±1.69），高于缓解组（2.45±0.69）和对照组（1.15±0.27）（均P<０.０５）。未治疗组IL-4水平为9.14%±4.34%，高于缓解组（4.78%±1.69%）和对照组（4.86%±1.41%）。未治疗组IFN-γ水平低于对照组（P<0.05）。Gata-３与pDC/mDC比值之间存在显著正相关（r=0.585，P<0.01）。Gata-３与IL-4之间存在显著正相关（r=0.463，P<0.05）。
ITP的发病机制可能与DC亚群紊乱及Gata-3高表达有关。
