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BSDF 抑制白色念珠菌黏附于导尿管。

BDSF inhibits Candida albicans adherence to urinary catheters.

机构信息

Laboratory of Advanced Materials, Fudan University, Shanghai 200433, PR China.

出版信息

Microb Pathog. 2013 Nov;64:33-8. doi: 10.1016/j.micpath.2013.07.003. Epub 2013 Aug 13.

Abstract

Cis-2-dodecenoic acid (BDSF) is a quorum-sensing signal molecule produced by the opportunistic pathogen Burkholderia cenocepacia and suppresses germ tube formation of Candida albicans. An in vitro model for biofilm formation evaluated the influence of BDSF on C. albicans. Biofilm morphology was observed using scanning electron microscopy, cell adherence was determined using polystyrene plates and siliconized urinary catheters, and the levels of expression of genes involved in adhesion were determined using Real-time Reverse-Transcriptase Polymerase Chain Reaction. BDSF inhibited initial biofilm formation by a clinical isolate of C. albicans and reduced its capability to adhere to the polystyrene surface. BDSF at concentrations up to 120 μM did not significantly affect the viability of C. albicans. BDSF (90 μM) inhibited cell adherence to plates and catheters by 4- and 25-fold. Compared with untreated yeasts, the level of expression of genes involved in adhesion, ALS1 and EAP1, were reduced by 4- and 0.25-fold, whereas that of YWP1 was increased at a 4-fold higher level. Here we show that BDSF effectively inhibited biofilm development as indicated by its ability to inhibit adherence. Thus, BDSF should be considered as a potential therapeutic agent to prevent disease caused by Candida species.

摘要

顺-2-十二烯酸(BDSF)是一种群体感应信号分子,由机会性病原体伯克霍尔德氏菌产生,并抑制白色念珠菌的芽管形成。体外生物膜形成模型评估了 BDSF 对白色念珠菌的影响。使用扫描电子显微镜观察生物膜形态,使用聚苯乙烯板和硅化尿导管测定细胞粘附,使用实时逆转录聚合酶链反应测定参与粘附的基因的表达水平。BDSF 抑制了白色念珠菌临床分离株的初始生物膜形成,并降低了其粘附聚苯乙烯表面的能力。高达 120μM 的 BDSF 对白色念珠菌的活力没有显著影响。BDSF(90μM)将细胞粘附到平板和导管上的抑制率分别为 4 倍和 25 倍。与未经处理的酵母相比,参与粘附的 ALS1 和 EAP1 基因的表达水平分别降低了 4 倍和 0.25 倍,而 YWP1 的表达水平则增加了 4 倍。在这里,我们表明 BDSF 能够有效抑制粘附,从而有效抑制生物膜的发展。因此,BDSF 可被视为预防念珠菌属引起的疾病的潜在治疗剂。

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