cis-2-十二烯酸对阴道念珠菌病实验小鼠模型的保护作用。

Protective Effects of cis-2-Dodecenoic Acid in an Experimental Mouse Model of Vaginal Candidiasis.

机构信息

Key Laboratory for Organic Electronics and Information Displays & Jiangsu Key Laboratory for Biosensors, Institute of Advanced Materials (IAM), Nanjing University of Posts and Telecommunications, Nanjing 210023, Jiangsu, China; School of Physical and Mathematical Sciences, Nanjing Tech University, Nanjing 211816, Jiangsu, China.

出版信息

Biomed Environ Sci. 2018 Nov;31(11):816-828. doi: 10.3967/bes2018.109.

DOI:10.3967/bes2018.109

PMID:30558702

Abstract

OBJECTIVE

To evaluate the efficacy of cis-2-dodecenoic acid (BDSF) in the treatment and prevention of vaginal candidiasis in vivo.

METHODS

The activities of different concentrations of BDSF against the virulence factors of Candida albicans (C. albicans) were determined in vitro. An experimental mouse model of Candida vaginitis was treated with 250 μmol/L BDSF. Treatment efficiency was evaluated in accordance with vaginal fungal burden and inflammation symptoms.

RESULTS

In vitro experiments indicated that BDSF attenuated the adhesion and damage of C. albicans to epithelial cells by decreasing phospholipase secretion and blocking filament formation. Treatment with 30 μmol/L BDSF reduced the adhesion and damage of C. albicans to epithelial cells by 36.9% and 42.3%, respectively. Treatment with 200 μmol/L BDSF completely inhibited phospholipase activity. In vivo mouse experiments demonstrated that BDSF could effectively eliminate vaginal infection and relieve inflammatory symptoms. Four days of treatment with 250 μmol/L BDSF reduced vaginal fungal loads by 6-fold and depressed inflammation. Moreover, BDSF treatment decreased the expression levels of the inflammatory chemokine-associated genes MCP-1 and IGFBP3 by 2.5- and 2-fold, respectively.

CONCLUSION

BDSF is a novel alternative drug that can efficiently control vaginal candidiasis by inhibiting the virulence factors of C. albicans.

摘要

目的

评估顺-2-十二烯酸（BDSF）在体内治疗和预防阴道念珠菌病的疗效。

方法

在体外测定不同浓度 BDSF 对白色念珠菌（C. albicans）毒力因子的活性。用 250 μmol/L BDSF 处理念珠菌阴道炎实验小鼠模型。根据阴道真菌负荷和炎症症状评估治疗效果。

结果

体外实验表明，BDSF 通过减少磷脂酶分泌和阻断丝状体形成来减弱 C. albicans 对上皮细胞的粘附和损伤。用 30 μmol/L BDSF 处理可分别使 C. albicans 对上皮细胞的粘附和损伤减少 36.9%和 42.3%。用 200 μmol/L BDSF 可完全抑制磷脂酶活性。体内小鼠实验表明，BDSF 可有效消除阴道感染并缓解炎症症状。用 250 μmol/L BDSF 治疗 4 天可使阴道真菌负荷减少 6 倍，并抑制炎症。此外，BDSF 处理使炎症趋化因子相关基因 MCP-1 和 IGFBP3 的表达水平分别降低了 2.5 倍和 2 倍。