Institute of Pesticide and Environmental Toxicology, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, China.
Chemosphere. 2014 Feb;96:51-6. doi: 10.1016/j.chemosphere.2013.07.016. Epub 2013 Aug 12.
Antibiotic persistence following five successive treatments of sulfadiazine (SDZ) and chlortetracycline (CTC), alone and in combination, in manure-amended soil was studied under laboratory conditions. The resulting effects on soil respiration and enzyme activities as well as pollution-induced community tolerance, were also examined. A trend of initial suppression followed by recovery was observed in the dissipation rates of SDZ or CTC during the antibiotic treatments, and combined treatment with both antibiotics did not alter the respective dissipation rates significantly. Soil respiration activity with SDZ and/or CTC treatments was inhibited during the initial two treatments; however, the activity thereafter recovered to or exceeded the level of the individual manure treatment. Initially, soil urease and dehydrogenase activities were not affected; however, after the fifth treatment, these activities were significantly stimulated in the CTC individual and combined treatments compared with their activities in the individual manure treatment. Bacterial community tolerance to SDZ and CTC in manure-amended soil increased significantly (p⩽0.05) with antibiotic treatment frequency.
在实验室条件下，研究了磺胺嘧啶（SDZ）和金霉素（CTC）连续 5 次单独和联合处理后在粪肥改良土壤中的抗生素持久性。还研究了对土壤呼吸和酶活性以及污染诱导的群落耐受性的影响。在抗生素处理过程中，SDZ 或 CTC 的消散率表现出初始抑制随后恢复的趋势，并且两种抗生素的联合处理并没有显著改变各自的消散率。在初始两次处理中，SDZ 和/或 CTC 处理抑制了土壤呼吸活性；然而，此后，活性恢复到或超过了单独粪肥处理的水平。最初，土壤脲酶和脱氢酶活性不受影响；然而，在第五次处理后，与单独粪肥处理相比，CTC 单独和联合处理的这些活性显著增加。粪肥改良土壤中细菌群落对 SDZ 和 CTC 的耐受性随着抗生素处理频率的增加而显著增加（p ⩽0.05）。
