Macau University of Science and Technology, Macau, China.
J Pharm Biomed Anal. 2013 Nov;85:186-93. doi: 10.1016/j.jpba.2013.07.026. Epub 2013 Jul 30.
We adopted an ultra performance liquid chromatography coupled with quadrupole time-of-fight mass spectrometry (UPLC Q-TOF/MS) metabonomics approach to study metabonomic features of rats induced by orally administered Pinellia ternata (Thunb.) Berit. (BX). The integrated urinary MS data were analyzed via principal component analysis (PCA) and partial least squares-discriminant analysis (OPLS-DA) to identify the differential metabolites. Ten potential biomarkers were identified within complex sample matrix of urine. The identified biomarkers indicated the perturbations of tryptophan, phenylacetylglycine and pantothenic acid metabolism in BX-induced rats. The biomarkers that were found to be changed with the passage of time were explained tentatively based on previous study.
我们采用超高效液相色谱-四极杆飞行时间质谱联用（UPLC-Q-TOF/MS）代谢组学方法研究了口服半夏（BX）诱导的大鼠代谢组学特征。通过主成分分析（PCA）和偏最小二乘判别分析（OPLS-DA）对整合的尿 MS 数据进行分析，以鉴定差异代谢物。在尿液复杂样本基质中鉴定出 10 个潜在的生物标志物。鉴定出的生物标志物表明 BX 诱导的大鼠色氨酸、苯乙酰甘氨酸和泛酸代谢受到干扰。根据先前的研究，对随时间变化的标志物进行了初步解释。
