Departments of Genetics, Demokritos University of Thrace, Alexandroupolis, Greece.
Cytotechnology. 2014 Oct;66(5):753-60. doi: 10.1007/s10616-013-9624-9. Epub 2013 Aug 15.
Ifosfamide (IFO) is an alkylating nitrogen mustard, administrated as an antineoplasmic agent. It is characterized by its intense urotoxic action, leading to hemorrhagic cystitis. This side effect of IFO raises the requirement for the co-administration with sodium 2-sulfanylethanesulfonate (Mesna) aiming to avoid or minimize this effect. IFO and Mesna were administrated separately on rabbit's lymphocytes in vivo, which were later developed in vitro. Cytogenetic markers for sister chromatid exchanges (SCEs), proliferation rate index (PRI) and Mitotic Index were recorded. Mesna's action, in conjunction with IFO reduces the frequency of SCEs, in comparison with the SCEs recordings obtained when IFO is administered alone. In addition to this, when high concentrations of Mesna were administered alone significant reductions of the PRI were noted, than with IFO acting at the same concentration on the lymphocytes. Mesna significantly reduces IFO's genotoxicity, while when administered in high concentrations it acts in an inhibitory fashion on the cytostatic action of the drug.
异环磷酰胺（IFO）是一种烷化氮芥，作为抗肿瘤药物使用。它的特点是具有强烈的尿毒性作用，导致出血性膀胱炎。IFO 的这种副作用要求与巯乙磺酸钠（Mesna）联合给药，以避免或最小化这种作用。IF0 和 Mesna 分别在体内培养的兔淋巴细胞上给药，然后在体外培养。姐妹染色单体交换（SCE）、增殖率指数（PRI）和有丝分裂指数的细胞遗传学标记被记录下来。Mesna 与 IFO 联合使用可降低 SCE 的频率，与单独使用 IFO 时的 SCE 记录相比。此外，当单独给予高浓度的 Mesna 时，PRI 显著降低，而当 IFO 以相同浓度作用于淋巴细胞时则没有。Mesna 显著降低了 IFO 的遗传毒性，而当高浓度给药时，它对药物的细胞抑制作用具有抑制作用。
