Lialiaris Theodoros, Mavromatidou Polyxeni, Digkas Evangelos, Passadaki Theoktisti, Mpountoukas Panagiotis, Panagoutsos Stylianos, Vargemezis Vassilios
Department of Genetics, Faculty of Medicine, Demokrition University of Thrace, Alexandroupolis, Greece.
Genet Test Mol Biomarkers. 2010 Feb;14(1):37-41. doi: 10.1089/gtmb.2009.0109.
The aim of this study was to investigate the frequency of sister chromatid exchanges (SCEs), the presence of cytostaticity, cytotoxicity, and therefore, the possible genetic instability in patients with chronic renal failure (CRF) in human cultured peripheral blood lymphocytes.
Peripheral blood lymphocytes were cultured from 32 patients with CRF (average 55.2 years) and 18 healthy blood donors (average 44.6 years), and the SCE method was applied afterward. The increase in SCE frequency was evaluated as an immediate DNA damage index, while the reduction in the values of the proliferating rate indices was evaluated as a cytostatic index and the mitotic indices as a cytotoxic index was also measured.
A significant increase in the SCE frequencies along with a significant reduction in mitotic indices was observed in patients with CRF compared with the controls. It is notable that there was no significant difference in SCE levels among patients with CRF and cancer, and patients with CRF alone.
This study illustrates increased genetic instability in patients with CRF. These results could also be of a great importance in early diagnosis to prognosticate a possible generation of neoplasm in the future.
本研究旨在调查慢性肾衰竭（CRF）患者外周血淋巴细胞中姐妹染色单体交换（SCE）的频率、细胞生长抑制性、细胞毒性以及可能存在的基因不稳定性。
从32例CRF患者（平均年龄55.2岁）和18例健康献血者（平均年龄44.6岁）中采集外周血淋巴细胞进行培养，随后采用SCE方法。SCE频率的增加被评估为即时DNA损伤指标，增殖率指标值的降低被评估为细胞生长抑制指标，同时也测量有丝分裂指标作为细胞毒性指标。
与对照组相比，CRF患者的SCE频率显著增加，有丝分裂指标显著降低。值得注意的是，CRF合并癌症患者与单纯CRF患者的SCE水平无显著差异。
本研究表明CRF患者的基因不稳定性增加。这些结果对于早期诊断未来可能发生的肿瘤也具有重要意义。
