Mukherjee A, Sarkar N, Barik A
Ecology Research Lab, Dept of Zoology, The Univ of Burdwan, Burdwan, 713 104, West Bengal, India.
Neotrop Entomol. 2013 Aug;42(4):366-71. doi: 10.1007/s13744-013-0137-1. Epub 2013 May 3.
Extraction, thin-layer chromatography, and gas chromatography-mass spectrophotometry analyses revealed 15 alkanes representing 97.14% of the total alkanes in the surface waxes of Momordica cochinchinensis Spreng flowers. Nonacosane was the prevailing alkane followed by hexatriacontane, nonadecane, heptacosane, and hentriacontane, accounting for 39.08%, 24.24%, 13.52%, 6.32%, and 5.12%, respectively. The alkanes from flower surface waxes followed by a synthetic mixture of alkanes mimicking alkanes of flower surface waxes elicited attraction of the female insect, Aulacophora foveicollis Lucas (Coleoptera: Chrysomelidae) between 2 and 10-μg/mL concentrations in a Y-shaped glass tube olfactometer bioassay under laboratory conditions. Synthetic nonadecane from 178.28-891.37 ng, heptacosane from 118.14-590.72 ng, and nonacosane at 784.73 ng showed attraction of the insect. A synthetic mixture of 534.82 ng nonadecane, 354.43 ng heptacosane, and 2,354.18 ng nonacosane elicited highest attraction of A. foveicollis.
提取、薄层色谱和气相色谱 - 质谱分析显示，在罗汉果花表面蜡质中，有15种烷烃，占总烷烃的97.14%。二十九烷是主要的烷烃，其次是三十六烷、十九烷、二十七烷和三十一烷，分别占39.08%、24.24%、13.52%、6.32%和5.12%。在实验室条件下的Y形玻璃管嗅觉测定生物测定中，花表面蜡质中的烷烃以及模拟花表面蜡质烷烃的合成烷烃混合物在2至10μg/mL浓度下引起了雌性昆虫黄斑蝽（鞘翅目：叶甲科）的吸引。178.28 - 891.37 ng的合成十九烷、118.14 - 590.72 ng的二十七烷和784.73 ng的二十九烷显示出对该昆虫的吸引。534.82 ng十九烷、354.43 ng二十七烷和2354.18 ng二十九烷的合成混合物引起了黄斑蝽的最高吸引。
