Fox C A, Adam D E, Watson R, Hoffman G E, Jacobson C D
Molecular, Cellular, and Developmental Biology Program, Iowa State University, Ames 50011.
J Neurobiol. 1990 Jul;21(5):705-18. doi: 10.1002/neu.480210505.
We have studied the anatomical localization of cholecystokinin-like immunoreactivity (CCK IR) in somata and fibers in the medial preoptic area (MPA) and anterior hypothalamus (AH) of the Brazilian gray short-tailed opossum, (Monodelphis domestica). With the aid of an avidin-biotin, nickel-enhanced, immunohistochemical technique, CCK IR neuronal elements were found within the MPA and AH. A large number of CCK IR cell bodies were located in the MPA of colchicine-treated opossums. The MPA also contained a CCK IR fiber plexus. Quantitative image analysis revealed that the periventricular preoptic area of noncolchicine-treated male opossums had a significantly higher percent of blocked light measurements than that of the noncolchicine-treated females, indicating a higher density of CCK IR neuronal elements in the males. Neuronal fibers and somata containing CCK IR were also found within the periventricular hypothalamic nucleus (Pe), and the suprachiasmatic nucleus (SCh). These results show that CCK IR neuronal elements are found within the MPA and AH of the Brazilian short-tailed opossum. Furthermore, there is a sexually dimorphic distribution of CCK IR elements within the MPA of this small marsupial.
我们研究了巴西灰短尾负鼠（Monodelphis domestica）内侧视前区（MPA）和下丘脑前部（AH）中胆囊收缩素样免疫反应性（CCK IR）在胞体和纤维中的解剖定位。借助抗生物素蛋白-生物素、镍增强免疫组织化学技术，在MPA和AH内发现了CCK IR神经元成分。在秋水仙碱处理的负鼠的MPA中发现了大量CCK IR细胞体。MPA中还存在CCK IR纤维丛。定量图像分析显示，未用秋水仙碱处理的雄性负鼠的室周视前区的遮光测量百分比显著高于未用秋水仙碱处理的雌性负鼠，表明雄性中CCK IR神经元成分的密度更高。在室周下丘脑核（Pe）和视交叉上核（SCh）内也发现了含有CCK IR的神经纤维和胞体。这些结果表明，在巴西短尾负鼠的MPA和AH内发现了CCK IR神经元成分。此外，在这种小有袋动物的MPA内，CCK IR成分存在性二态分布。
