Institute of Forensic Sciences, Ankara University, No: 27, Dikimevi, 06590, Ankara, Turkey,
Aging Clin Exp Res. 2013 Oct;25(5):511-6. doi: 10.1007/s40520-013-0129-3. Epub 2013 Aug 15.
Aging is a complex and multifactorial process that is stimulated by a number of factors including genes and life-style. It is thought that the production of reactive oxygen species (ROS) in the face of antioxidant enzymes and molecules is related to aging and age-related diseases. NAD(P)H oxidase system is the predominant cellular source of ROS, and p22phox, the major component of that system, is essential for the activation of NAD(P)H oxidase. The aim of this study was to investigate the association between p22phox C242T single nucleotide polymorphism and aging in Turkish population.
Blood samples were collected from 332 volunteers between 18 and 95 years of age and were classified into three groups according to their ages as <65, 65-84 and ≥ 85. p22phox C242T polymorphism was genotyped by PCR-RFLP method.
CC genotype frequency in the C242T polymorphism is higher in older group (≥ 85) than younger groups (<65 and 65-85), whereas CT + TT genotype frequency is lower in older group. When the p22phox C242T polymorphism was compared with the mean ages and age groups, statistically significant associations were found.
We showed for the first time that human aging is significantly associated with p22phox C242T genotypes in Turkish population, being highest in CC, intermediate in CT, and lowest in TT homozygote. It is plausible to suggest that CC genotype might protect people from chronic inflammation, diseases as well as from oxidative stress and, thus, individuals with CC genotype might be more advantageous for aging as compared to those with CT + TT genotypes.
衰老是一个复杂的多因素过程，受多种因素的刺激，包括基因和生活方式。人们认为，面对抗氧化酶和分子，活性氧（ROS）的产生与衰老和与年龄相关的疾病有关。NAD（P）H 氧化酶系统是 ROS 的主要细胞来源，该系统的主要成分 p22phox 对于 NAD（P）H 氧化酶的激活是必不可少的。本研究的目的是探讨土耳其人群中 p22phox C242T 单核苷酸多态性与衰老的关系。
从 18 至 95 岁的 332 名志愿者中采集血液样本，并根据年龄将其分为<65、65-84 和≥85 三个年龄组。采用 PCR-RFLP 法检测 p22phox C242T 多态性。
C242T 多态性中的 CC 基因型频率在老年组（≥85 岁）高于年轻组（<65 岁和 65-85 岁），而 CT + TT 基因型频率在老年组较低。当 p22phox C242T 多态性与平均年龄和年龄组进行比较时，发现存在统计学显著关联。
我们首次表明，在土耳其人群中，人类衰老与 p22phox C242T 基因型显著相关，CC 基因型最高，CT 基因型次之，TT 纯合子最低。可以合理地假设，CC 基因型可能保护人们免受慢性炎症、疾病以及氧化应激的影响，因此，与 CT + TT 基因型相比，CC 基因型的个体可能更有利于衰老。
