NADPH氧化酶p22phox基因的C242T多态性降低了埃及人群随机样本中冠状动脉疾病的风险。
C242T polymorphism of NADPH oxidase p22phox gene reduces the risk of coronary artery disease in a random sample of Egyptian population.
作者信息
Hashad Ingy M, Abdel Rahman Mohamed F, Abdel-Maksoud Sahar M, Amr Khalda S, Effat Laila K, Shaban Gamal M, Gad Mohamed Z
机构信息
Clinical Biochemistry Unit, Faculty of Pharmacy and Biotechnology, The German University in Cairo, New Cairo City, Al Tagamoa Al Khames, Cairo, 11835, Egypt.
出版信息
Mol Biol Rep. 2014;41(4):2281-6. doi: 10.1007/s11033-014-3081-1. Epub 2014 Jan 11.
The p22phox protein subunit is essential for NADPH oxidase activity. The prevalence of C242T variants of p22phox gene was studied in 101 healthy Egyptian controls and 104 acute myocardial infarction (AMI) Egyptian patients. Contribution of oxidative stress, represented by serum oxidized-LDL (ox-LDL), in development of AMI was also examined and correlated with C242T gene variants. Genotyping and ox-LDL were assessed by PCR-RFLP and ELISA. Results showed that wild type CC genotype is prevalent in 27 % of controls; CT and TT are in 72 and 1 %. In patients, the distribution was 40.2, 59.8 and 0 % for CC, CT and TT; respectively, showing a significant difference (p = 0.0259). Serum ox-LDL levels were higher in patients than controls (p ≤ 0.0001). Subjects having CT genotype had lower levels of ox-LDL than CC genotype (p ≤ 0.005). C242T polymorphism of p22phox gene of NADPH oxidase is a novel genetic marker associated with reduced susceptibility to AMI.
p22phox蛋白亚基对于NADPH氧化酶活性至关重要。在101名健康埃及对照者和104名急性心肌梗死（AMI）埃及患者中研究了p22phox基因C242T变异的发生率。还检测了以血清氧化型低密度脂蛋白（ox-LDL）为代表的氧化应激在AMI发生中的作用，并将其与C242T基因变异相关联。通过聚合酶链反应-限制性片段长度多态性（PCR-RFLP）和酶联免疫吸附测定（ELISA）评估基因分型和ox-LDL。结果显示，野生型CC基因型在27%的对照者中占优势；CT和TT分别占72%和1%。在患者中，CC、CT和TT的分布分别为40.2%、59.8%和0%，显示出显著差异（p = 0.0259）。患者的血清ox-LDL水平高于对照者（p≤0.0001）。具有CT基因型的受试者的ox-LDL水平低于CC基因型（p≤0.005）。NADPH氧化酶p22phox基因的C242T多态性是一种与AMI易感性降低相关的新型遗传标志物。
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