多个瞬时受体电位通道基因在鼠 3T3-L1 细胞系和脂肪组织中的表达。
Expression of multiple Transient Receptor Potential channel genes in murine 3T3-L1 cell lines and adipose tissue.
机构信息
National Agri-Food Biotechnology Institute (NABI), SAS Nagar (Mohali)-160071, Punjab, India.
出版信息
Pharmacol Rep. 2013;65(3):751-5. doi: 10.1016/s1734-1140(13)71055-7.
BACKGROUND
Calcium and its signaling have a role in adipogenesis. Transient Receptor Potential (TRP) channels are non-selective cation channels with a high permeability to calcium.
METHODS
In the present study the expression of multiple TRP channels on mouse 3T3-L1 preadipocyte and adipocyte cells, white (WAT) and brown (BAT) adipose tissues was investigated using real time PCR (RT-PCR).
RESULTS
TRPV1, TRPV3, TRPM8, TRPC4, TRPC6 were differentially expressed in preadipocytes and adipocytes suggesting their significance in adipogenesis. Genes for multiple TRP channels were also expressed in murine WAT and BAT, out of which TRPV4, TRPV6 and TRPC6 showed differential expression.
CONCLUSION
Present study demonstrates the expression of TRP channels in mouse cell lines and adipose tissues.
背景
钙及其信号在脂肪生成中起作用。瞬时受体电位 (TRP) 通道是非选择性阳离子通道，对钙具有高通透性。
方法
本研究使用实时 PCR (RT-PCR) 研究了多个 TRP 通道在小鼠 3T3-L1 前体脂肪细胞和脂肪细胞、白色 (WAT) 和棕色 (BAT) 脂肪组织中的表达。
结果
TRPV1、TRPV3、TRPM8、TRPC4 和 TRPC6 在前体脂肪细胞和脂肪细胞中的表达不同，表明它们在脂肪生成中的重要性。多个 TRP 通道的基因也在鼠 WAT 和 BAT 中表达，其中 TRPV4、TRPV6 和 TRPC6 表现出差异表达。
结论
本研究表明 TRP 通道在小鼠细胞系和脂肪组织中的表达。
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