Sun Wuping, Yu Zhijian, Yang Shaomin, Jiang Changyu, Kou Yanbo, Xiao Lizu, Tang Shuo, Zhu Tao
Department of Pain Medicine and Shenzhen Municipal Key Laboratory for Pain Medicine, Shenzhen Nanshan People's Hospital and The 6th Affiliated Hospital of Shenzhen University Health Science Center, Shenzhen, China.
Department of Infectious Diseases and Shenzhen Municipal Key Laboratory for Endogenous Infection, Shenzhen Nanshan People's Hospital and The 6th Affiliated Hospital of Shenzhen University Health Science Center, Shenzhen, China.
Front Mol Biosci. 2020 Sep 16;7:564339. doi: 10.3389/fmolb.2020.564339. eCollection 2020.
Obesity is characterized by increased adipose tissue mass that results from increased fat cell size (hypertrophy) and number (hyperplasia). The molecular mechanisms that govern the regulation and differentiation of adipocytes play a critical role for better understanding of the pathological mechanism of obesity. However, the mechanism of adipocyte differentiation is still unclear.
The present study aims to compare the gene expression changes during adipocyte differentiation in the transcriptomic level, which may help to better understand the mechanism of adipocyte differentiation.
RNA sequencing (RNA-seq) technology, GO and KEGG analysis, quantitative RT-PCR, and oil red O staining methods were used in this study.
A lot of genes were up- or down-regulated between each two differentiation stages of 3T3-L1 cells. Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis revealed that lipid metabolism and oxidation-reduction reaction were mainly involved in the whole process of adipocyte differentiation. Decreased immune response and cell cycle adhesion occurred in the late phase of adipocyte differentiation, which was demonstrated by divergent expression pattern analysis. Moreover, quantitative RT-PCR results showed that the mRNA expression levels of , , , and were significantly decreased in the differentiated adipocytes. On the other hand, the mRNA expression levels of , , , and were significantly increased in the differentiated adipocytes. Besides, the mRNA expressions of TRPV2 and TRPM7 were also significantly increased in subcutaneous white adipose tissue from diet-induced mice. In addition, the activation of TRPM7, TRPV1, and TRPV2 suppressed the differentiation of adipocytes.
These data present the description of transcription profile changes during adipocyte differentiation and provides an in-depth analysis of the possible mechanisms of adipocyte differentiation. These data offer new insight into the understanding of the mechanisms of adipocyte differentiation.
肥胖的特征是脂肪组织量增加，这是由脂肪细胞大小增加（肥大）和数量增加（增生）导致的。控制脂肪细胞调节和分化的分子机制对于更好地理解肥胖的病理机制起着关键作用。然而，脂肪细胞分化的机制仍不清楚。
本研究旨在比较转录组水平上脂肪细胞分化过程中的基因表达变化，这可能有助于更好地理解脂肪细胞分化的机制。
本研究采用RNA测序（RNA-seq）技术、GO和KEGG分析、定量RT-PCR以及油红O染色方法。
在3T3-L1细胞的每两个分化阶段之间，许多基因上调或下调。基因本体论（GO）和京都基因与基因组百科全书（KEGG）分析表明，脂质代谢和氧化还原反应主要参与脂肪细胞分化的全过程。脂肪细胞分化后期免疫反应和细胞周期黏附降低，这通过差异表达模式分析得到证实。此外，定量RT-PCR结果显示，在分化的脂肪细胞中， 、 、 和 的mRNA表达水平显著降低。另一方面，在分化的脂肪细胞中， 、 、 和 的mRNA表达水平显著升高。此外，饮食诱导小鼠皮下白色脂肪组织中TRPV2和TRPM7的mRNA表达也显著增加。此外，TRPM7、TRPV1和TRPV2的激活抑制了脂肪细胞的分化。
这些数据描述了脂肪细胞分化过程中转录谱的变化，并对脂肪细胞分化的可能机制进行了深入分析。这些数据为理解脂肪细胞分化机制提供了新的见解。
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