大肠杆菌DNA结合蛋白的体内和体外磷酸化作用
In vivo and in vitro phosphorylation of DNA-binding proteins from Escherichia coli.
作者信息
Kurek E, Dominik K
出版信息
Acta Microbiol Pol A. 1975;7(2):87-97.
PMID:239529
Abstract
A deoxyribonucleoprotein (DNP) complex has been isolated from Escherichia coli cells by chromatography on Sephadex G-200. The DNP complex contains phosphoproteins and the content of phosphorus bound to the DNP protein is 3 times higher than in cytoplasmic proteins not bound to DNA. These results have been confirmed by in vivo (32-P-KH2PO4) and in vitro (32-P-ATP) phosphorylation of E. coli DNA-binding proteins isolated by chromatography on DNA--cellulose.
摘要
通过在葡聚糖凝胶G - 200上进行色谱分离，从大肠杆菌细胞中分离出了一种脱氧核糖核蛋白（DNP）复合物。该DNP复合物含有磷蛋白，与DNP蛋白结合的磷含量比未与DNA结合的细胞质蛋白中的磷含量高3倍。通过对经DNA - 纤维素色谱分离得到的大肠杆菌DNA结合蛋白进行体内（32 - P - KH2PO4）和体外（32 - P - ATP）磷酸化，这些结果得到了证实。
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