Kulyba N P, Kozlov A V
Biokhimiia. 1989 Jul;54(7):1075-81.
The possibility of quantitative determination of protein HU in E. coli cell lysates was demonstrated, using enzyme immunoassay with monospecific polyclonal antibodies against HU and homogeneous protein HU. The protein HU/DNA ratio in two cultures of E. coli with different generation periods was found to be constant despite the differences in the levels of proteins HU and DNA. Protein HU was found to be represented by approximately 420.10(3) copies per fast growing. E. coli cell and by 320.10(3) copies per slowly growing cell. These amounts of the HU protein correspond to a ratio of one protein HU molecule per 44 +/- 8 base pairs of DNA and can provide for the nucleosome-like organization of approximately 30% of E. coli DNA length. The constant HU/DNA ratio which is similar to constant histones/DNA ratio provides additional evidence in favour of functional similarity of the HU E. coli DNA-binding protein to histones.
利用针对HU的单特异性多克隆抗体和纯蛋白HU进行酶免疫测定，证明了定量测定大肠杆菌细胞裂解物中蛋白质HU的可能性。尽管蛋白质HU和DNA的水平存在差异，但在两个不同代时的大肠杆菌培养物中，蛋白质HU/DNA的比率是恒定的。发现快速生长的大肠杆菌细胞中，蛋白质HU约以420×10³个拷贝存在，而缓慢生长的细胞中则以320×10³个拷贝存在。这些HU蛋白的量相当于每44±8个DNA碱基对有一个HU蛋白分子，并且可以使大约30%的大肠杆菌DNA长度形成类似核小体的结构。与恒定的组蛋白/DNA比率相似的恒定HU/DNA比率，为大肠杆菌DNA结合蛋白HU与组蛋白的功能相似性提供了额外证据。
