Department of Pathology, Ann and Robert H. Lurie Children's Hospital of Chicago, Feinberg School of Medicine, Northwestern University, Chicago, IL 60611, USA.
Am J Clin Pathol. 2013 Sep;140(3):293-300. doi: 10.1309/AJCPLSN0RQKHJX1A.
Recent reports have provided conflicting evidence on the stability of CCR3 expression on the surface of basophils. Hence we wanted to independently evaluate the diagnostic usefulness of CCR3 as a surrogate marker of basophil activation and function.
We examined the correlative relationship between CCR3 expression on the surface of donor basophils and histamine release after donor basophils were treated with agonistic antibodies directed against the high-affinity IgE-Fc receptor and serum samples from 80 individuals displaying symptoms of chronic urticaria (CU).
We observed that CCR3 was significantly downregulated on donor basophils treated with the agonistic antibody and CU-patient serum that demonstrated positive "histamine-releasing activity" (HRA scores >10). However, CCR3 downregulation was also observed on donor basophils incubated with more than 40% of CU-patient serum samples with HRA scores less than or equal to 10.
Overall our data show that CCR3 demonstrates adequate sensitivity (83%) but weak specificity (59%) in its ability to reliably identify histamine-releasing activated basophils.
最近的报告提供了相互矛盾的证据，表明嗜碱性粒细胞表面 CCR3 表达的稳定性。因此，我们希望独立评估 CCR3 作为嗜碱性粒细胞激活和功能替代标志物的诊断有用性。
我们研究了供体嗜碱性粒细胞表面 CCR3 表达与供体嗜碱性粒细胞用针对高亲和力 IgE-Fc 受体的激动性抗体和来自 80 名患有慢性荨麻疹 (CU) 症状个体的血清样本处理后组胺释放之间的相关性。
我们观察到，用激动性抗体和 CU 患者血清处理的供体嗜碱性粒细胞中 CCR3 明显下调，这些 CU 患者血清表现出阳性“组胺释放活性”（HRA 评分>10）。然而，在与 HRA 评分小于或等于 10 的超过 40%的 CU 患者血清样本孵育的供体嗜碱性粒细胞中也观察到 CCR3 下调。
总的来说，我们的数据表明 CCR3 在可靠识别释放组胺的激活嗜碱性粒细胞方面具有足够的敏感性（83%）但特异性较弱（59%）。
