Key Laboratory of Microbial Diversity in Southwest China, Ministry of Education, and Laboratory for Conservation and Utilization of Bio-resources, Yunnan Institute of Microbiology, Yunnan University, Kunming, 650091, People's Republic of China.
Antonie Van Leeuwenhoek. 2013 Nov;104(5):837-43. doi: 10.1007/s10482-013-9996-z. Epub 2013 Aug 17.
A Gram-positive, aerobic, non-motile actinobacterial strain, designated YIM C01235(T), was isolated from a soil sample collected from the Swallow Cave, Yunnan province, south-west China. The isolate grew at 10-30 °C, pH 6.0-9.0 and 0-8 % (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences revealed highest similarity to Saccharopolyspora gloriosae YIM 60513(T) (96.8 %), and lower 16S rRNA gene sequence similarities (95.1-96.7 %) with the other species of the genus Saccharopolyspora. The whole-cell hydrolysates contained meso-diaminopimelic acid (meso-DAP), arabinose and galactose. The polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, phosphatidylmethylethanolamine, two unknown glycolipids, two unknown phospholipids and one polar lipid. MK-9(H4) was the predominant menaquinone. The major fatty acids were iso-C15:0, iso-C16:0, anteiso-C15:0 and anteiso-C17:0. Mycolic acids were not detected. The genomic DNA G+C content was 69.1 mol%. These chemotaxonomic data, together with its morphological properties, were consistent with the assignment of strain YIM C01235(T) to the genus Saccharopolyspora. The results of physiological and biochemical tests allowed strain YIM C01235(T) to be differentiated phenotypically from all the recognized Saccharopolyspora species. On the basis of evidence from this polyphasic study, the novel species Saccharopolyspora cavernae sp. nov. is proposed. The type strain is YIM C01235(T) (=DSM 45825(T) = CCTCC AA 2012022(T)).
一株革兰氏阳性、需氧、不运动放线菌菌株，被命名为 YIM C01235(T)，从中国西南部云南省燕子洞的土壤样本中分离得到。该分离株在 10-30°C、pH6.0-9.0 和 0-8%（w/v）NaCl 的条件下生长。基于 16S rRNA 基因序列的系统发育分析显示，与 Saccharopolyspora gloriosae YIM 60513(T)（96.8%）的相似度最高，与 Saccharopolyspora 属的其他种的 16S rRNA 基因序列相似度较低（95.1-96.7%）。全细胞水解物含有 meso-二氨基庚二酸（meso-DAP）、阿拉伯糖和半乳糖。极性脂类为双磷脂酰甘油、磷脂酰甘油、磷脂酰胆碱、磷脂酰乙醇胺、磷脂甲乙醇胺、两种未知糖脂、两种未知磷脂和一种极性脂类。MK-9(H4) 是主要的menaquinone。主要脂肪酸为 iso-C15:0、iso-C16:0、anteiso-C15:0 和 anteiso-C17:0。未检测到分枝菌酸。基因组 DNA G+C 含量为 69.1 mol%。这些化学分类学数据，加上其形态学特性，与菌株 YIM C01235(T)被归为 Saccharopolyspora 属一致。生理生化试验的结果表明，菌株 YIM C01235(T)在表型上可与所有已识别的 Saccharopolyspora 种区分开来。基于多相研究的证据，提出了一个新的物种 Saccharopolyspora cavernae sp. nov.。该模式株为 YIM C01235(T)（=DSM 45825(T)=CCTCC AA 2012022(T)）。
