The Key Laboratory of Biotechnology for Medicinal Plant of Jiangsu Province, School of Life Science, Jiangsu Normal University, Xuzhou, 221116, Jiangsu, People's Republic of China.
Antonie Van Leeuwenhoek. 2013 Jun;103(6):1369-76. doi: 10.1007/s10482-013-9917-1. Epub 2013 Apr 5.
A halotolerant actinomycete strain, designated strain KLBMP 1305(T), was isolated from a salt marsh plant Dendranthema indicum (Linn.) Des Moul collected from the coastal region of Nantong, Jiangsu Province, in east China and was studied in detail for its taxonomic position. Phylogenetic analysis based on the 16S rRNA gene sequence revealed that strain KLBMP 1305(T) is a member of the genus Saccharopolyspora. The 16S rRNA gene sequence similarity indicated that strain KLBMP 1305(T) was most closely related to 'Saccharopolyspora pathumthaniensis' S582(T) (99.31 %), 'Saccharopolyspora endophytica' YIM 61095(T) (99.17 %) and Saccharopolyspora tripterygii YIM 65359(T) (99.15 %); similarity to other type strains of the genus Saccharopolyspora was <97.2 %. The organism had chemical and morphological features consistent with its classification in the genus Saccharopolyspora such as meso-diaminopimelic acid as the diagnostic diamino acid in the cell wall peptidoglycan and arabinose and galactose as the diagnostic sugars. The predominant menaquinone was MK-9(H4). The polar lipids detected were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, an unknown glycolipid and an unknown lipid. The major fatty acids were iso-C16:0, iso-C15:0, anteiso-C15:0, anteiso-C17:0 and sum in feature 8 (18:1ω7c/18:1ω6c). The G+C content of the genomic DNA of the type strain was 68.7 mol%. DNA-DNA relatedness data, together with phenotypic differences, clearly distinguished the isolate from its closest relatives. On the basis of these phenotypic and genotypic data, the isolate represents a novel species, for which the name Saccharopolyspora dendranthemae sp. nov. is proposed. The type strain is KLBMP 1305(T) (=KCTC 19889(T) = NBRC 108675(T)).
一株耐盐放线菌菌株，命名为 KLBMP 1305(T)，从中国东部江苏省南通市沿海地区采集的盐沼植物菊花(Dendranthema indicum (Linn.) Des Moul)中分离得到，并对其分类地位进行了详细研究。基于 16S rRNA 基因序列的系统发育分析表明，菌株 KLBMP 1305(T)是糖多孢菌属的一员。16S rRNA 基因序列相似性表明，菌株 KLBMP 1305(T)与 'Saccharopolyspora pathumthaniensis' S582(T) (99.31 %)、'Saccharopolyspora endophytica' YIM 61095(T) (99.17 %)和 Saccharopolyspora tripterygii YIM 65359(T) (99.15 %)最为密切相关；与糖多孢菌属其他模式菌株的相似性<97.2 %。该菌具有化学和形态特征，与糖多孢菌属的分类一致，如细胞壁肽聚糖中的二氨基庚二酸为特征二氨基庚二酸，阿拉伯糖和半乳糖为特征糖。主要的类异戊二烯醌为 MK-9(H4)。检测到的极性脂为双磷脂酰甘油、磷脂酰甘油、磷脂酰乙醇胺、磷脂酰胆碱、一种未知的糖脂和一种未知的脂。主要脂肪酸为 iso-C16:0、iso-C15:0、anteiso-C15:0、anteiso-C17:0 和特征 8 (18:1ω7c/18:1ω6c)中的总和。模式菌株的基因组 DNA 的 G+C 含量为 68.7 mol%。DNA-DNA 同源性数据以及表型差异清楚地区分了该分离株与其最接近的亲缘关系。基于这些表型和基因型数据，该分离株代表一个新种，建议命名为菊花糖多孢菌(Saccharopolyspora dendranthemae sp. nov.)。模式菌株为 KLBMP 1305(T)=KCTC 19889(T)=NBRC 108675(T)。
