Bartsch F, Kang M L, Mees S T, Haier J, Gassmann P
General, Visceral and Transplantation Surgery, Medical Center of the Johannes Gutenberg-University Mainz, Mainz, Germany.
Methods Mol Biol. 2013;1066:89-101. doi: 10.1007/978-1-62703-604-7_8.
In vivo and ex vivo fluorescence video microscopy used to be a well-established method in life science with a variety of applications, such as in inflammation or cancer research. In this book chapter, we describe a model of in vivo fluorescence microscopy of the rat's lung with the exclusive advantage of qualitative and quantitative in vivo analysis of cell adhesion within the complex microenvironment of the ventilated and perfused lung. Observation can include real-time, time-lapse, or fast-motion analysis. In our laboratory, we have used the model for qualitative and quantitative real-time analyses of metastatic colon cancer cell adhesion within the rat's pulmonary microcirculation. Using some modifications in another series, we have also applied the model to analyze thrombocyte and leucocyte adhesion within the pulmonary capillaries in experimental sepsis. For interventional studies, injected cells or animals may be pretreated with various reagents or drugs for further analysis of adhesion molecules involved in tumor cell-endothelial cell interactions.
体内和体外荧光视频显微镜检查曾经是生命科学中一种成熟的方法，有多种应用，比如在炎症或癌症研究中。在本章中，我们描述了一种大鼠肺脏体内荧光显微镜检查模型，其独特优势在于能够对通气和灌注肺脏的复杂微环境中的细胞黏附进行定性和定量的体内分析。观察可以包括实时、延时或快动作分析。在我们实验室，我们已将该模型用于对大鼠肺微循环中转移性结肠癌细胞黏附进行定性和定量实时分析。在另一系列实验中，通过一些改进，我们还将该模型应用于分析实验性脓毒症时肺毛细血管内血小板和白细胞的黏附情况。对于干预性研究，可对注射的细胞或动物用各种试剂或药物进行预处理，以进一步分析参与肿瘤细胞 - 内皮细胞相互作用的黏附分子。
