Zhang Jian, Guo Wei-Hui, Rape Andrew, Wang Yu-Li
Department of Biomedical Engineering, Carnegie Mellon University, Pittsburgh, PA, USA.
Methods Mol Biol. 2013;1066:147-56. doi: 10.1007/978-1-62703-604-7_13.
Cell shape and substrate rigidity play critical roles in regulating cell behaviors and fate. Controlling cell shape on elastic adhesive materials holds great promise for creating a physiologically relevant culture environment for basic and translational research and clinical applications. However, it has been technically challenging to create high-quality adhesive patterns on compliant substrates. We have developed an efficient and economical method to create precise micron-scaled adhesive patterns on the surface of a hydrogel (Rape et al., Biomaterials 32:2043-2051, 2011). This method will facilitate the research on traction force generation, cellular mechanotransduction, and tissue engineering, where precise controls of both materials rigidity and adhesive patterns are important.
细胞形状和底物硬度在调节细胞行为和命运方面起着关键作用。在弹性粘附材料上控制细胞形状，对于为基础研究、转化研究及临床应用创建生理相关的培养环境具有巨大潜力。然而，在柔性底物上创建高质量的粘附图案在技术上一直具有挑战性。我们已经开发出一种高效且经济的方法，可在水凝胶表面创建精确的微米级粘附图案（Rape等人，《生物材料》32:2043 - 2051，2011年）。该方法将促进对牵张力产生、细胞机械转导和组织工程的研究，其中对材料硬度和粘附图案的精确控制都很重要。
