Luchina N N, Shostak N G, Timofeeva M Ia
Ontogenez. 1990 May-Jun;21(3):261-6.
Relative content of the oocyte and somatic 5S rRNA in loach Misgurnus fossilis L. during development was determined electrophoretically. Embryos before hatching contain 70% and swimming larvae no less than 50% of the oocyte 5S rRNA. We assume that the relative content of 5S rRNA fractions reflects the proportion between ribosomes synthesized during oogenesis and those synthesized in embryos and larvae. We calculated using previous data (Timofeeva, Kafiani, 1964) the rates of maternal ribosome decay and ribosome synthesis in the embryo. During organogenesis these rates appear to be 1.17-1.09 x 10(6) and 1.7 x 10(6) molecules/sec per embryo, respectively.
通过电泳测定了泥鳅（Misgurnus fossilis L.）发育过程中卵母细胞和体细胞5S rRNA的相对含量。孵化前的胚胎含有70%的卵母细胞5S rRNA，游动幼虫含有的卵母细胞5S rRNA不少于50%。我们假设5S rRNA组分的相对含量反映了卵子发生过程中合成的核糖体与胚胎和幼虫中合成的核糖体之间的比例。我们利用先前的数据（Timofeeva，Kafiani，1964）计算了胚胎中母体核糖体的降解速率和核糖体合成速率。在器官发生过程中，这些速率分别为每个胚胎1.17 - 1.09×10⁶和1.7×10⁶分子/秒。
