Krigsgaber M R, Neifakh A A, Madatova N P
Ontogenez. 1976;7(1):47-57.
The synthesis of ribosomal proteins (r-proteins) in the loach embryos was studied by the way of analysis of protein-labeled ribosomes isolated from the cytoplasm and purified from de novo synthesized polypeptide and contaminating proteins. The ribosomes at the mid-blastula and mid-gastrula stages were shown to contain no de novo synthesized proteins. The synthesis of r-proteins at the stage of organogenesis was revealed by sedimentation analysis of ribosomes in the gradient of sucrose concentrations, density analysis in the gradient of cesium chloride and analysis of the distribution of radioactivity in r-proteins separated from the labelled ribosomes by electrophoresis in polyacrilamide gel. At the stage of organogenesis, the exchange of ribosomal proteins with de novo synthesized r-proteins continues under the conditions of complete inhibition of RNA synthesis and incubation of unlabeled ribosomes with the labelled cytosol. The correlation between the absence of rRNA and r-protein syntheses at the early developmental stages and possible causes of the independence of r-protein synthesis and exchange from the simultaneous rRNA synthesis at the stage of organogenesis are discussed.
通过对从细胞质中分离并从新合成的多肽和污染蛋白中纯化的蛋白质标记核糖体进行分析，研究了泥鳅胚胎中核糖体蛋白（r蛋白）的合成。囊胚中期和原肠胚中期的核糖体显示不含有新合成的蛋白质。通过在蔗糖浓度梯度中对核糖体进行沉降分析、在氯化铯梯度中进行密度分析以及对通过聚丙烯酰胺凝胶电泳从标记核糖体中分离出的r蛋白中的放射性分布进行分析，揭示了器官发生阶段r蛋白的合成。在器官发生阶段，在RNA合成完全抑制以及未标记核糖体与标记细胞质孵育的条件下，核糖体蛋白与新合成的r蛋白继续进行交换。讨论了早期发育阶段rRNA和r蛋白合成缺失之间的相关性，以及器官发生阶段r蛋白合成和交换与同时进行的rRNA合成无关的可能原因。
