Töz Seray Ozensoy, Ertabaklar Hatice, Göçmen Bayram, Demir Samiye, Karakuş Mehmet, Arserim Suha Kenan, Balcıoğlu I Cüneyt, Canakçı Tayfun, Ozbel Yusuf
Ege Üniversitesi Tıp Fakültesi, Parazitoloji Anabilim Dalı, İzmir, Turkey.
Turkiye Parazitol Derg. 2013;37(2):107-12. doi: 10.5152/tpd.2013.25.
In this study, the investigation on the prevalence of canine leishmaniasis (CanL) and sand fly species incriminated as potential vectors of leishmaniasis in the northern part of the Cyprus were aimed.
This research was conducted in two periods; 2004 and 2012. Serological (IFAT and rK39) and molecular (PCR) tests were performed on 83 dog blood samples during the 2004 survey. PCR was performed using primers 13A/13B targeting kinetoplastid minicircle constant region. Genomic Nested-PCR was applied using R221/R332 and R323/333 primers for 5 clinically suspected dog samples in 2012. Sand flies were collected from the Lapithos town and Kyreniae province using CDC light traps and midgut dissection was done for the presence of Leishmania parasites during the 2012 survey.
Three (3.61%) out of 83 dogs were found to be positive for CanL in 2004, while 3 out of 5 clinically suspected dogs were positive in 2012. In total 296 female sand flies were dissected and 9 species belonging to Phlebotomus and Sergentomyia genera were determined. No promastigote was found in the dissected females.
The results obtained in two different periods showed that the importance and risk of canine disease are increasing in the northern Cyprus and further studies should be performed in northern Cyprus for determining the incidence of canine and human leishmaniasis.
本研究旨在调查塞浦路斯北部犬利什曼病（CanL）的流行情况以及被认为是利什曼病潜在传播媒介的白蛉种类。
本研究分两个阶段进行，分别为2004年和2012年。在2004年的调查中，对83份犬血样本进行了血清学检测（间接荧光抗体试验和rK39）和分子检测（聚合酶链反应）。聚合酶链反应使用靶向动基体小环恒定区的引物13A/13B。2012年，对5份临床疑似犬样本应用R221/R332和R323/333引物进行基因组巢式聚合酶链反应。在2012年的调查中，使用疾控中心诱蚊灯从拉皮索斯镇和凯里尼亚省采集白蛉，并对其进行中肠解剖以检测利什曼原虫寄生虫的存在。
2004年，83只犬中有3只（3.61%）被检测出犬利什曼病呈阳性，而2012年，5只临床疑似犬中有3只呈阳性。总共解剖了296只雌性白蛉，确定了属于白蛉属和司蛉属的9个种类。在解剖的雌性白蛉中未发现前鞭毛体。
两个不同时期获得的结果表明，塞浦路斯北部犬类疾病的重要性和风险正在增加，应在塞浦路斯北部进行进一步研究，以确定犬类和人类利什曼病的发病率。
