College of Plant Protection, Henan Agricultural University.
Insect Sci. 2014 Aug;21(4):459-68. doi: 10.1111/1744-7917.12053. Epub 2013 Oct 4.
Cytoplasmic lipid droplet (LD) lipolysis is regulated by pheromone biosynthesis activating neuropeptide (PBAN) in Bombyx mori. To elucidate the molecular mechanism of cytoplasm LD lipolysis, the pancreatic lipase-like gene in B. mori pheromone glands (PGs), designated as B. mori pancreatic lipase-like gene (BmPLLG), was identified in this study. Spatial expression analysis revealed that BmPLLG is a ubiquitous gene present in all studied tissues, such as PGs, brain, epidermis, egg, midgut, flight muscle and fat body. Temporal expression analysis showed that the BmPLLG transcript begins to express 96 h before eclosion (-96 h), continues to increase, peaks in newly emerged females and steadily decreases after eclosion. Translational expression analysis of BmPLLG using a prepared antiserum demonstrated that BmPLLG was expressed in an age-dependent pattern at different development stages in B. mori. This finding was similar to the transcript expression pattern. Further RNA interference-mediated knockdown of BmPLLG significantly inhibited bombykol production. Overall, these results demonstrated that BmPLLG is involved in PBAN-induced sex pheromone biosynthesis and release.
细胞质脂滴 (LD) 的脂解受家蚕信息素生物合成激活神经肽 (PBAN) 调节。为了阐明细胞质 LD 脂解的分子机制，本研究在家蚕香腺中鉴定了一种胰腺脂肪酶样基因，命名为家蚕胰腺脂肪酶样基因 (BmPLLG)。空间表达分析显示，BmPLLG 是一种普遍存在的基因，存在于所有研究的组织中，如香腺、脑、表皮、卵、中肠、飞行肌和脂肪体。时间表达分析表明，BmPLLG 转录本在蜕皮前 96 小时 (-96 h) 开始表达，持续增加，在新羽化的雌虫中达到峰值，羽化后稳定下降。使用制备的抗血清进行 BmPLLG 的翻译表达分析表明，BmPLLG 在不同发育阶段的年龄依赖性模式中在家蚕中表达。这一发现与转录本表达模式相似。进一步的 RNAi 介导的 BmPLLG 敲低显著抑制了 bombykol 的产生。总体而言，这些结果表明 BmPLLG 参与了 PBAN 诱导的性信息素生物合成和释放。
