[Screening of chlorobenzene-degrading bacterium and purification of its degradation enzyme].

OBJECTIVE

We screened a bacterial strain capable of degrading chlorobenzene, and purified the corresponding degradation enzyme.

METHODS

The strain was screened by gradient enrichment culture and sterile filter paper plate method, and identified by morphology and 16S rRNA gene sequence. Chlorobenzene concentration in the liquid culture was determined by gas chromatography. Degradation capability was assayed by the proportion of chlorobenzene degraded per cells. The purity quotient and molecular weight of the purified degradation enzyme were determined by gel per cell. electrophoresis.

RESULTS

The isolated bacterium, LW13, used chlorobenzene in activated sludge as sole carbon and energy source. Cells were 2.3 microm long and 0.8 microm wide, with several terminal flagella. Strain LW13 was 95.5% similar to Lysinibacillus fusiformis, and its degradation enzyme was a positively-charged exoenzyme (molecular weight about 57 kDa). The optimal temperature and pH of the purified enzyme were approximately 40 degrees C and 8.0, respectively.

CONCLUSION

Strain LW13 belongs to genus Lysinibacillus, and can degrade chlorobenzene (500-2000 mg/L).