Ye Jie-Xu, Lin Tong-Hui, Luo Yu-Hao, Chen Dong-Zhi, Chen Jian-Meng
College of Environment, Zhejiang University of Technology, Hangzhou 310032, China.
Huan Jing Ke Xue. 2017 Feb 8;38(2):802-808. doi: 10.13227/j.hjkx.201607198.
A bacterium strain LW26 which could utilize chlorobenzene (CB) as sole carbon and energy source was isolated from a biotrickling filter reactor treating CB-contaminated off-gas. Based on its morphological and physiological characteristics, as well as the analysis of 16S rRNA gene sequence and Biolog test, the strain LW26 was identified as . To our best knowledge, it is the first time that the strain was applied for CB purification. In this study, the effects of temperature, pH, initial CB concentration and Cl concentration on the biodegradation were investigated. The results showed that the optimal temperature and pH for CB biodegradation were 25℃ and 7.0,respectively; the maximum CB tolerated concentration for LW26 was as high as 500 mg·L; when the concentration of Cl was above 0.14 mol·L, the CB degradation was significantly restrained. The degrading process of the strain LW26 followed the Haldane kinetic model and the maximum specific growth rate and the maximum specific degradation rate were 0.42 h and 2.53 h, respectively.GC-MS analysis of the metabolites revealed that CB was firstly converted to o-chlorophenol by strain LW26. Combined with the activity of catechol dioxygenase, it can be speculated that CB was finally mineralized to CO, or converted to cell biomass after processes of ortho cleavage,dechlorination and oxidation.
从处理含氯苯废气的生物滴滤反应器中分离出一株能以氯苯（CB）作为唯一碳源和能源的细菌菌株LW26。基于其形态和生理特征，以及16S rRNA基因序列分析和Biolog测试，菌株LW26被鉴定为 。据我们所知，这是该菌株首次应用于氯苯净化。本研究考察了温度、pH值、初始氯苯浓度和氯离子浓度对生物降解的影响。结果表明，氯苯生物降解的最佳温度和pH值分别为25℃和7.0；菌株LW26耐受氯苯的最大浓度高达500 mg·L；当氯离子浓度高于0.14 mol·L时，氯苯降解受到显著抑制。菌株LW26的降解过程符合Haldane动力学模型，最大比生长速率和最大比降解速率分别为0.42 h和2.53 h。代谢产物的GC-MS分析表明，菌株LW26首先将氯苯转化为邻氯苯酚。结合儿茶酚双加氧酶的活性，可以推测氯苯最终被矿化为CO，或经过邻位裂解、脱氯和氧化过程后转化为细胞生物量。
