Institute of Dermatology, Taiyuan City Center Hospital, Taiyuan, China.
Department of Dermatology, Affiliated Hospital of Qinghai University, Xining, China.
J Am Acad Dermatol. 2013 Nov;69(5):e235-e243. doi: 10.1016/j.jaad.2013.06.030. Epub 2013 Aug 16.
Psoriasis, lichen planus (LP), and atopic dermatitis (AD) are common chronic inflammatory skin diseases mediated by immune responses.
We used RNA sequencing to investigate messenger RNA expression patterns in peripheral T cells of Chinese patients with psoriasis, LP, or AD and of healthy individuals.
After peripheral T-cell proliferation, messenger RNA expression patterns were investigated by RNA sequencing, and 6 randomly selected genes were verified by real-time reverse transcriptase polymerase chain reaction.
Six genes were down-regulated and 33 were up-regulated in these diseases. Gene ontology analysis revealed enrichment of genes involved in positive regulation of T-cell activation. Regulation of nuclear premessenger RNA domain containing 1B (RPRD1B) expression was enhanced in psoriasis.
The role of hereditary factors in RPRD1B expression in T cells was not considered. Immunomodulators (thymopeptide, levamisole, BCG polysaccharide, nucleic acid injection, and transfer factor) were previously given to patients with psoriasis and LP, but not to patients with AD; the effects of these immunomodulators on gene expression is uncertain.
RPRD1B may be involved in T-cell activation in our Chinese psoriatic cohort, and may play a role in stimulating epidermal hyperproliferation.
银屑病、扁平苔藓（LP）和特应性皮炎（AD）是由免疫反应介导的常见慢性炎症性皮肤病。
我们使用 RNA 测序研究了中国银屑病、LP 或 AD 患者和健康个体外周 T 细胞中的信使 RNA 表达模式。
外周 T 细胞增殖后，通过 RNA 测序研究信使 RNA 表达模式，并通过实时逆转录聚合酶链反应验证 6 个随机选择的基因。
这些疾病中，有 6 个基因下调，33 个基因上调。基因本体分析显示，参与 T 细胞激活的正调控的基因富集。银屑病中核前信使 RNA 结构域包含 1B（RPRD1B）表达的调节增强。
未考虑遗传因素对 T 细胞中 RPRD1B 表达的作用。免疫调节剂（胸腺肽、左旋咪唑、卡介苗多糖、核酸注射和转移因子）以前曾用于银屑病和 LP 患者，但不适用于 AD 患者；这些免疫调节剂对基因表达的影响尚不确定。
RPRD1B 可能参与我们中国银屑病患者的 T 细胞激活，并可能在刺激表皮过度增殖中发挥作用。
