食源性沙门氏菌的定量分析——小型改良半固体Rappaport Vassiliadis最大可能数法研究

[Quantitative analysis of foodborne salmonella-the study of mini-modified semi solid rappaport vassiliadis most probable number method].

作者信息

Zhang Ding, Liao Xing-guang, Guo Yun-chang, Zhang Xiu-li, Chuan Hui-xia, Cui Ying

机构信息

Henan Center for Disease Control and Prevention, Zhengzhou, China.

出版信息

Zhonghua Yu Fang Yi Xue Za Zhi. 2013 May;47(5):452-4.

PMID:23958131

Abstract

OBJECTIVE

To improve the mini-modified semi solid rappaport vassiliadis most probable number (mini-MSRV MPN) method for Salmonella detection.

METHODS

Based on the mini-MSRV MPN method,Buffered Peptone Water (BPW) was modified as one step enrichment medium and Modified Semi Solid Rappaport Vassiliadis (MSRV) medium was ameliorated as modified MSRV for Salmonella detection under standard Salmonella addition recovery. A total of 154 raw chicken samples, 48 swabs of pheasantry and 48 poultry dung samples were collected to compare the detection results of Salmonella by using improved mini-MSRV MPN, mini-MSRV MPN and regular most probable number (MPN) method.

RESULTS

Salmonella recovery was < 2.7 MPN/g when the standard Salmonella addition was at the concentration of 0.9 CFU/g when the mini-MSRV MPN method was employed. If the standard Salmonella addition were at 9.0 and 90.0 CFU/g, the recoveries of bacteria were 10.1 and 94.0 MPN/g, and the average recovery rate was 112% and 104%, respectively. Salmonella detection rate of modified mini-MSRV MPN, mini-MSRV MPN and regular MPN method was 18.4% (46/250), 5.2% (13/250) and 6.0% (15/250), respectively. The detection rate was higher for modified mini-MSRV MPN method than of the other two methods (χ(2) values were 19.68 and 17.82, respectively, all P values < 0.05). The detection quantity of Salmonella (medians were 21.0, < 2.7 and < 3.0 MPN/g, respectively). The quantity detected by modified mini-MSRV MPN method was higher than that of the other two methods (both Z values were 5.71, both P values < 0.05).

CONCLUSION

Modified mini-MSRV MPN method is an accurate method for foodborne Salmonella detection.

摘要

目的

改进用于沙门氏菌检测的小型改良半固体拉帕波特-瓦西利亚迪斯最大可能数（mini-MSRV MPN）法。

方法

基于mini-MSRV MPN法，将缓冲蛋白胨水（BPW）改良为一步增菌培养基，将改良半固体拉帕波特-瓦西利亚迪斯（MSRV）培养基改良为改良MSRV，用于在标准添加沙门氏菌回收条件下检测沙门氏菌。共采集154份生鸡肉样品、48份野鸡拭子和48份家禽粪便样品，采用改良的mini-MSRV MPN法、mini-MSRV MPN法和常规最大可能数（MPN）法比较沙门氏菌的检测结果。

结果

采用mini-MSRV MPN法时，当标准添加沙门氏菌浓度为0.9 CFU/g时，沙门氏菌回收率<2.7 MPN/g。若标准添加沙门氏菌浓度为9.0和90.0 CFU/g，细菌回收率分别为10.1和94.0 MPN/g，平均回收率分别为112%和104%。改良mini-MSRV MPN法、mini-MSRV MPN法和常规MPN法的沙门氏菌检出率分别为18.4%（46/250）、5.2%（13/250）和6.0%（15/250）。改良mini-MSRV MPN法的检出率高于其他两种方法（χ²值分别为19.68和17.82，P值均<0.05）。沙门氏菌的检测量（中位数分别为21.0、<2.7和<3.0 MPN/g）。改良mini-MSRV MPN法检测的量高于其他两种方法（Z值均为5.71，P值均<0.05）。