Institute of Pharmaceutical Sciences, ETH Zurich, Zurich, Switzerland.
Eur J Pharm Biopharm. 2013 Sep;85(1):139-42. doi: 10.1016/j.ejpb.2013.03.030.
Axonal repair and regeneration remain critical due to lack of appropriate delivery systems for efficient release of neurotrophic factors (NTFs). Recently, we have demonstrated the synergistic activity of nerve growth factor (NGF) and glial cell line-derived neurotrophic factor (GDNF) on axonal regeneration. Combined delivery of GDNF and NGF with individually controlled release kinetics may be crucial for exploiting their synergistic action on axonal elongation in animals. For engineering discrete NTF release kinetics, we have developed several nerve conduits (NCs) using collagen (Col) and silk fibroin (SF); the NC were made of Col or SF alone, or of Col and SF layers, or of Col/SF blends, all loaded with GDNF and NGF. All NC types provided sustained combined release of NGF and GDNF over 28 days. NC made of combinations of Col and SF showed reduced burst and more sustained dual release of GDNF and NGF. SF/Col-based NC scaffolds provide an adaptable delivery system for growth factors and hold potential for nerve regeneration and possibly for other tissue engineering applications.
由于缺乏有效的递药系统来高效释放神经营养因子（NTFs），轴突修复和再生仍然是至关重要的。最近，我们已经证明了神经生长因子（NGF）和胶质细胞系源性神经营养因子（GDNF）在轴突再生方面的协同作用。联合递送 GDNF 和 NGF 并结合单独控制的释放动力学，对于在动物中利用它们在轴突伸长方面的协同作用可能是至关重要的。为了实现离散 NTF 释放动力学的工程化，我们使用胶原（Col）和丝素蛋白（SF）开发了几种神经导管（NC）；NC 由 Col 或 SF 单独制成，或者由 Col 和 SF 层制成，或者由 Col/SF 混合物制成，所有 NC 都负载有 GDNF 和 NGF。所有 NC 类型在 28 天内均提供了 NGF 和 GDNF 的持续联合释放。由 Col 和 SF 组合而成的 NC 显示出较低的爆发和更持续的 GDNF 和 NGF 的双重释放。SF/Col 基 NC 支架提供了一种适应性强的生长因子递药系统，具有神经再生的潜力，并且可能在其他组织工程应用中具有潜力。
