Department of Civil and Environmental Engineering, University of Windsor, 401 Sunset Ave., Essex Hall, Windsor, Ontario N9B 3P4, Canada.
Department of Civil and Environmental Engineering, University of Windsor, 401 Sunset Ave., Essex Hall, Windsor, Ontario N9B 3P4, Canada; Great Lakes Institute of Environmental Research, University of Windsor, 401 Sunset Ave., Essex Hall, Windsor, Ontario N9B 3P4, Canada.
Bioresour Technol. 2013 Oct;146:775-778. doi: 10.1016/j.biortech.2013.07.125. Epub 2013 Jul 31.
In this study, a flux balance analysis (FBA) was adopted to estimate the activity of acetogenic H2-consuming reaction. Experimental data at different substrate concentrations of 10, 20, and 30 g COD/L showing the lowest, medium, and highest H2 yields, respectively, were used in the FBA to calculate the fluxes. It was interesting to note that the hydrogenase activity based on R12 (2Fd(+)+2H(+)→2Fd(2+)+H2, ferredoxin (Fd)) flux was most active at 10 g COD/L. The flux of R17 (4H2+2CO2→CH3COOH), a mechanism for reutilizing produced H2, increased in steps of 0.030, 0.119, and 0.467 as the substrate concentration decreased. Contradictory to our general understanding, acetate production found to have a negligible or even negative effect on the final H2 yield in dark fermentation.
在这项研究中，采用通量平衡分析（FBA）来估计产乙酸菌消耗 H2 的反应活性。将分别具有最低、中等和最高 H2 产率的 10、20 和 30 g COD/L 等三种不同底物浓度的实验数据用于 FBA 计算通量。有趣的是，基于 R12（2Fd(+)+2H(+)→2Fd(2+)+H2，铁氧还蛋白（Fd））通量的氢化酶活性在 10 g COD/L 时最为活跃。重新利用产生的 H2 的机制 R17（4H2+2CO2→CH3COOH）的通量随着底物浓度的降低分别以 0.030、0.119 和 0.467 的步长增加。与我们的普遍理解相反，在黑暗发酵中，乙酸盐的产生对最终 H2 产率几乎没有影响，甚至有负面影响。
