Reznikov A G, Akmaev I G, Fidelina O F, Gorbatiuk O S, Goufman E I, Gusev A A, Kalimullina L B, Pivnitskiĭ K K, Sergeev V G, Vikhreva O V
Probl Endokrinol (Mosk). 1990 May-Jun;36(3):57-61.
Experiments in vitro with further thin-layer chromatography of steroids were staged to investigate changing of 3H-testosterone into 3H-estradiol-17 beta and 3H-5 alpha-reduced metabolites in a fraction of 1000 g of homogenates of the medial preoptic area (MPA), the paraventricular nuclei (PVN), the dorsal vagal complex (DVC) of the lower cerebral trunk, the rostral and caudal parts of the amygdaloid complex (ACr and ACc) of the brain of 21-day male and female rat fetuses. The activity of aromatase (AA) and 5 alpha-reductase (AR) was detected in all investigated areas. AA was maximum in PVN of females and minimum in ACc of males and females. The production of 5 alpha-dehydrotestosterone prevailed among 5 alpha-reduced products in PVN, DVC and ACr, 5 alpha- and rostane-3 alpha and 17 beta-diol--in MPA and ACr, AR in ACr was much higher than in ACc. AR in DVC of females was slightly higher than in males. Sex differences in the other cerebral areas were undetectable. The results of the investigation suggest that the above cerebral structures are dependent on sex hormones.
进行了类固醇的进一步薄层色谱体外实验，以研究在21日龄雄性和雌性大鼠胎儿大脑的内侧视前区（MPA）、室旁核（PVN）、脑桥下部的迷走背核复合体（DVC）、杏仁核复合体的嘴侧和尾侧部分（ACr和ACc）的1000g匀浆部分中3H-睾酮向3H-雌二醇-17β和3H-5α-还原代谢产物的变化。在所有研究区域均检测到芳香化酶（AA）和5α-还原酶（AR）的活性。AA在雌性的PVN中最高，在雄性和雌性的ACc中最低。在PVN、DVC和ACr中，5α-还原产物中5α-脱氢睾酮的生成占主导，在MPA和ACr中为5α-和罗烷-3α和17β-二醇，ACr中的AR远高于ACc。雌性DVC中的AR略高于雄性。在其他脑区未检测到性别差异。研究结果表明，上述脑结构依赖于性激素。
