Kazanova E G, Smirnov A N, Rozen V B
Probl Endokrinol (Mosk). 1990 May-Jun;36(3):66-72.
Hormone-binding and some other physicochemical properties of an estrogen-binding protein (EBP) from the female and male rat pancreas in partially purified specimens were studied. Kinetic parameters of the hormone-protein interaction were found to be the same for males and females. However the concentration of binding sites for estradiol in females was higher than that in males. The female and male EBP exhibited nearly the same specificity for hormonal compounds except for some estrogens and androgens, whose competitive efficiency was higher in males. These sex differences were found not to be ascribed to diverse intensity of hormonal metabolism during incubation with male and female EBP specimens. The data obtained are suggestive of sex differentiation in the quantity and/or quality of low molecular "accessory" factor modifying EBP hormone-binding properties.
研究了部分纯化标本中来自雌性和雄性大鼠胰腺的雌激素结合蛋白（EBP）的激素结合及其他一些物理化学性质。发现激素与蛋白质相互作用的动力学参数在雄性和雌性中是相同的。然而，雌性中雌二醇结合位点的浓度高于雄性。除了一些雌激素和雄激素外，雌性和雄性EBP对激素化合物表现出几乎相同的特异性，而这些雌激素和雄激素在雄性中的竞争效率更高。这些性别差异并非归因于与雄性和雌性EBP标本孵育期间激素代谢强度的不同。所获得的数据表明，在修饰EBP激素结合特性的低分子“辅助”因子的数量和/或质量上存在性别差异。
