Krug A
Am J Cardiol. 1975 Aug;36(2):214-7. doi: 10.1016/0002-9149(75)90529-9.
Alterations of hydrogen ion (H+) concentration in the myocardium of 60 cats were determined before release of coronary ligation of 30, 45, 60, 90 or 180 minutes' duration, or 5, 30 and 90 minutes after release of the occlusion, by pressing pH indicator paper on unfixed frozen heart sections. The H+ concentration had clearly increased (pH less than 6.0) in a transmural extension in the left ventricle before release of the coronary ligature. This elevated H+ concentration persisted some time in the subendocardial region despite release of the coronary occlusion, thus proving that the so-called no reflow phenomenon exists in involved myocardium after temporary coronary occlusion. A diminished H+ concentration (pH 7.4) was found in the border zone between the acid-reacting area and the normal muscle when the coronary occlusion lasted 180 minutes before release of the ligature or 30 minutes after release of the occlusion when the previous period of temporary occlusion had been longer than 30 minutes. Although areas manifesting an acid reaction vanished or diminished in size after release of coronary occlusion, the extent of alkaline-reacting zones showed no reduction in size, and muscle cell necrosis developed in these alkaline-reacting areas. It is assumed therefore that an alkaline reaction of the myocardium is an early sign of irreversible muscle cell damage.
通过将pH试纸按压在未固定的冷冻心脏切片上，测定了60只猫在冠状动脉结扎30、45、60、90或180分钟后松开结扎之前，或在松开结扎后5、30和90分钟时心肌中氢离子（H+）浓度的变化。在冠状动脉结扎松开之前，左心室透壁区域的H+浓度明显升高（pH值小于6.0）。尽管冠状动脉阻塞已解除，但这种升高的H+浓度在内膜下区域持续了一段时间，从而证明在冠状动脉暂时阻塞后，受累心肌中存在所谓的无再流现象。当冠状动脉阻塞在松开结扎前持续180分钟，或在松开结扎后30分钟（如果之前的暂时阻塞时间超过30分钟）时，在酸反应区和正常心肌之间的边界区域发现H+浓度降低（pH值为7.4）。尽管冠状动脉阻塞解除后，表现出酸反应的区域消失或面积减小，但碱反应区的范围并未减小，并且在这些碱反应区域发生了肌肉细胞坏死。因此可以推测，心肌的碱反应是肌肉细胞不可逆损伤的早期迹象。
