Biochemical Processing and Biomaterial Research Laboratory, Gazi University, 06500 Teknikokullar, Ankara, Turkey; Department of Chemistry, Gazi University, 06500 Teknikokullar, Ankara, Turkey.
J Chromatogr B Analyt Technol Biomed Life Sci. 2013 Oct 1;936:1-9. doi: 10.1016/j.jchromb.2013.07.025. Epub 2013 Jul 31.
A novel core shell beaded chromatographic materials was prepared by grafting of glycidyl methacrylate (GMA) on to the surface of poly(hydoxypropyl methacrylate/ethyleneglycol dimethacrylate), p(HPMA/EGDMA) beads via surface-initiated atom transfer radical polymerization (SI-ATRP). For grafting GMA, p(HPMA/EGDMA) beads were first modified with an ATRP initiator. A reaction with 2-bromo-2-methylpropionyl bromide of the hydroxyl groups of the beads led to ATRP initiator-covered surfaces. The grafted p(GMA) fibrous chains on the beads were decorated with two different ligands (i.e., Protein L and l-histidine) for separation of Immunoglobulin's (Igs) from aqueous solution in batch system. The maximum Igs adsorptions on the p(HPMA/EGDMA)-g-p(GMA)-Protein L and p(HPMA/EGDMA)-g-p(GMA)-l-histidine affinity beads were found to be 81.8 and 112.3mg/g at pH 7.5 and 5.5, respectively. The purity of Igs from human serum was analyzed by HPLC. The Protein L immobilized affinity beads provided purity about 98%. The novel core shell polymeric beads decorated with Protein L showed a good selectivity for Igs molecules from diluted human serum. Adsorption studies of Igs onto Protein L and l-histidine immobilized affinity beads were also carried out in a continuous system.
一种新型核壳珠状色谱材料是通过表面引发原子转移自由基聚合（SI-ATRP）将甲基丙烯酸缩水甘油酯（GMA）接枝到聚（羟丙基甲基丙烯酸酯/乙二醇二甲基丙烯酸酯），p（HPMA/EGDMA）珠表面上制备的。为了接枝 GMA，首先用 ATRP 引发剂对 p（HPMA/EGDMA）珠进行修饰。珠粒上的羟基与 2-溴-2-甲基丙酰溴反应，导致 ATRP 引发剂覆盖的表面。珠上接枝的 p（GMA）纤维链用两种不同的配体（即蛋白 L 和 l-组氨酸）进行修饰，用于在批处理系统中从水溶液中分离免疫球蛋白（Igs）。在 pH 7.5 和 5.5 下，p（HPMA/EGDMA）-g-p（GMA）-Protein L 和 p（HPMA/EGDMA）-g-p（GMA）-l-histidine 亲和珠对 Igs 的最大吸附量分别为 81.8 和 112.3mg/g。通过 HPLC 分析人血清中 Igs 的纯度。固定化蛋白 L 的亲和珠提供了约 98%的纯度。用蛋白 L 修饰的新型核壳聚合物珠对稀释人血清中的 Igs 分子表现出良好的选择性。还在连续系统中进行了 Igs 吸附到蛋白 L 和 l-组氨酸固定化亲和珠上的研究。
