Shakrin N N S M, Balasubramaniam S D, Yusof H A, Mastuki M F, Masri S N, Taib N M, Nordin S A, Jamal F, Clarke S C, Desa M N M
Department of Biomedical Sciences, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia.
Trop Biomed. 2013 Jun;30(2):338-44.
Determination of Streptococcus pneumoniae serotypes is essential for epidemiological surveillance. Therefore accurate, reliable and cost effective serotyping method is crucial. In this study, we determined the serotypes of 41 pneumococcal isolates recovered from human anterior nares by multiplex Polymerase Chain Reaction (PCR) utilizing published primers. The data was then compared with conventional serology using latex agglutination (LA) and the Quellung reaction. Based on the PCR-approach, 8 different serogroups/serotypes were detected with one isolate classified as non-typeable (cpsA-negative). In reference to the serology-based data, the results were in agreement except for one isolate. For the latter isolate, the LA and Quellung tests failed to show a reaction but the PCR-approach and sequencing identified the isolate as serogroup 15B/C. Based on this experimental setting, we found that the PCR-approach for pneumococcal serotypes determination is reliable to serve as the alternative for determining the pneumococcal serotyping.
肺炎链球菌血清型的确定对于流行病学监测至关重要。因此，准确、可靠且具有成本效益的血清分型方法至关重要。在本研究中，我们利用已发表的引物，通过多重聚合酶链反应（PCR）确定了从人类前鼻孔分离出的41株肺炎球菌分离株的血清型。然后将数据与使用乳胶凝集（LA）和荚膜肿胀反应的传统血清学方法进行比较。基于PCR方法，检测到8种不同的血清群/血清型，其中1株分离株被分类为不可分型（cpsA阴性）。参照基于血清学的数据，除1株分离株外，结果一致。对于后一株分离株，LA和荚膜肿胀试验未显示反应，但PCR方法和测序将该分离株鉴定为血清群15B/C。基于此实验设置，我们发现用于肺炎球菌血清型确定的PCR方法可可靠地作为确定肺炎球菌血清分型的替代方法。
